牛肝菌提取液在细胞水平的抗衰老功效研究

Study of anti-aging efficacy of bolete at cell theory level

通过水提取法分离提取得到牛肝菌提取液,生药质量浓度为60 g/L。采用XTT比色法检测牛肝菌提取液对表皮角质细胞的线粒体活性、观察细胞增殖并检测其对细胞DNA含量的影响;利用DCFH-DA等方法检测牛肝菌提取液对人皮肤成纤维细胞内活性氧(ROS)及抗氧化元件(ARE)的影响;利用免疫荧光法检测牛肝菌提取液对成纤维细胞Ⅰ型前胶原蛋白表达的影响。结果表明:牛肝菌提取液在终质量浓度为1.5 g/L时,较对照组可以显著提升角质细胞的线粒体活性及DNA的含量,能够明显促进细胞的增殖;牛肝菌提取液可以明显减少细胞内ROS的含量、提高细胞内ARE激活水平;同时牛肝菌提取液还可以促进Ⅰ型前胶原蛋白的表达。

Bolete extract with mass concentration of 60 g/L was obtained by water extraction method. Effects of bolete in the extract on mitochondrial activity of epidermal keratinous cells were detected with XTT eolorimetry, as well as cell proliferation observed and effect on cellular DNA content of cell detected. Effects of bolete in the extract on cellular ROS content of human dermal fibroblast and ARE activation were detected with DCFH - DA assay, et al. Effects of bolete in the extract on expression of procollagen of type I in human dermal fibroblast was detected with immuno - fluorescence method. Results showed that the extract of bolete with mass concentration of 1.5 g/L can significantly increase the mitochondrial activity of epidermal keratinous cells and cellular DNA content as comparing with that of the control group and can apparently promote proliferation of cell. Further, ROS content in human dermal fibroblast is significantly reduced and ARE activation is promoted. Meanwhile, the extract of bolete can promote the expression of procollagen of type I too.