悬浮固化液相微萃取-高效液相色谱法分析生长素

Analysis of auxins by solidification of floating organic drop combined with high-performance liquid chromatography

建立基于悬浮固化液相微萃取预处理的高效液相色谱-荧光检测法（SFODME-HPLC-FLD）用于两种植物生长素（吲哚丙酸和吲哚丁酸）的分析.实验考察各因素对萃取过程的影响,确定最佳条件：50μL十二醇（纯）为萃取剂,20.0 m L样品溶液（p H 3.0）、0.25 g·m L-1Na Cl、26℃、1 800 r·min-1条件下萃取10 min.结果表明,SFODME技术重现性好、灵敏度高,相对标准偏差小于8.66%,检测限为0.01 ng·m L-1,线性范围为0.02~60 ng·m L-1,加标回收率大于84.3%.方法快速、稳定、环境友好,适用于实际样品的检测.

Determination of two anxins（ indole propionic acid and indole butyric acid） by solidification of floated organic drop microextraction with high- performance liquid chromatography- fluorescence detection（ SFODME- HPLC- FLD） was established. The experimental conditions affecting the extraction process were optimized. The optimal conditions of SFODME were as follows： 50 μL dodecanol as extraction solvent,20. 0 m L sample solution with p H 3. 0 and 0. 25 g · m L- 1Na Cl. The above solution was extracted for 10 min at temperature of 26 ℃ and centrifugation speed of 1 800r·min- 1. Results showed that this SFODME approach had high sensitivity and good reproducibility,with relative standard deviations lower than 8. 66%. Its linear range was 0. 02 ~ 60 ng·m L- 1,with the limit of detection of 0. 01 ng · m L- 1and the recoveries larger than 84. 3%. This method was rapid,stable and environmentally benign,and it can be applied to real sample analysis.