不同浓度神经肽P物质刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响

Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat

本研究旨在探讨不同浓度的神经肽P物质(SP)刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响。本研究分别采用0(对照组)、1×10-6、1×10-7、1×10-8、1×10-9 mol/L 5个浓度梯度的SP对内蒙古白绒山羊皮肤干细胞进行刺激处理,并分别于第0、7、14及21天采用实时荧光定量PCR方法鉴定BMP2和BMP4基因的表达量。结果显示,1×10-6和1×10-8 mol/L的SP促进BMP2、BMP4的表达;在7和14d时,SP1×10-8 mol/L组BMP2的表达量显著高于SP 1×10-6 mol/L组(P<0.05),SP 1×10-6 mol/L组BMP4的表达量与SP 1×10-8 mol/L组差异不显著(P>0.05);在21d时,SP 1×10-8 mol/L、SP 1×10-6 mol/L组BMP2、BMP4的表达量都明显降低,其中SP 1×10-8 mol/L组BMP2的表达量显著高于1×10-6 mol/L组(P<0.05),SP1×10-6 mol/L组BMP4的表达量与SP 1×10-8 mol/L组差异不显著(P>0.05)。结果表明,SP 1×10-6和1×10-8 mol/L不仅对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因的表达量有影响,而且为皮肤干细胞的定向分化提供理论基础。

This study was aimed to explore the expression of BMP2 and BMP4genes in skin stem cells of Mongolia Cashmere goat with the influence of different concentrations of neuropeptide P（SP）.We set five different SP concentrations（0,1×10^-6,1×10^-7,1×10^-8 and 1×10^-9 mol/L）to stimulate the skin stem cells,then identified the expression quantities of BMP2 and BMP4at days of 0,7,14 and 21by Real-time quantitative PCR with the purpose of determining the optimum SP concentration for the differentiation of skin stem cells.The results showed that SP with the concentrations of 1×10^-6 and 1 ×10^-8 mol/L promoted the expression of BMP2 and BMP4;The expression of BMP2 at 7and 14 din SP 1×10^-8 mol/L group was significantly higher than that of the SP 1×10^-6 mol/L group（P〈0.05）;The expression of BMP4 in SP 1×10^-6 mol/L group had no significant difference with the SP 1×10^-8 mol/L group（P〈0.05）;At 21 d,the expression of BMP2 and BMP4significantly decreased in SP 1×10^-8 and the SP 1×10^-6 mol/Lgroups,and the expression of BMP2 in the SP 1×10^-8 mol/L group was significantly higher than that of the 1×10^-6 mol/L group（P 〈0.05）,while the expression of BMP4 in the SP 1×10^-6 mol/L group had no significant difference with that of SP 1×10^-8 mol/L group（P〈0.05）.The results indicated that the concentrations of 1×10^-6 and 1×10^-8 mol/L not only might affect the expression of BMP2 and BMP4of skin stem cells,but also provided a theoretical basis for the directional differentiation of skin stem cells.