摘要
为研究大黄对猪流行性腹泻病毒(PEDV)感染所引起的细胞病变的抑制作用及其机理,本试验采用形态学观察法测定大黄溶液在Vero细胞上的最大安全浓度,建立体外病毒感染Vero细胞模型并利用该模型进行了大黄对病毒抑制作用的研究。结果显示,本试验成功建立了体外病毒感染模型和大黄的抗病毒试验方法;大黄在Vero细胞上的最大安全浓度为3.28mg/mL;形态学观察法和MTT法均显示大黄不仅能阻断病毒吸附Vero细胞,抑制病毒的合成复制,还可直接灭活病毒。本研究结果表明,大黄具有抗PEDV的作用,为临床上预防和治疗猪流行性腹泻(PED)提供理论基础。
The inhibitory effects of rhubarb on cell lesion,which was caused by porcine epidemic diarrhea virus（PEDV）,and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation,and then the PEDV infected Vero cell model in vitro was established,by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was3.28mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus,but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV,and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第9期2474-2480,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家863计划专项基金(新型抗感染功能型微生态制剂的研制与应用)(2013AA102806)
