不同频率重复经颅磁刺激对慢性应激抑郁模型大鼠行为的影响

Effect of different frequency repetitive transcranial magnetic stimulation on depression-like behavior for chronic stress depression model rats

目的:观察慢性应激抑郁模型大鼠经不同频率的重复经颅磁刺激(rTMS)干预后行为学的变化。方法:40只成年雄性SD大鼠分为正常对照组(n=8)和造模组(n=32)。造模组大鼠采用孤养联合慢性不可预见性温和应激方法制备抑郁模型后分为4组:模型组(n=8,不给予任何处理)、高频组(n=8,给予10 Hz的rTMS干预)、低频组(n=8,给予1 Hz的r TMS干预)和伪刺激组(n=8,模拟rTMS环境但不发出rTMS刺激)。在rTMS干预结束后进行行为学评估,包括测量体重、蔗糖水消耗实验以及旷场实验。结果:5组大鼠的体重减分率、蔗糖水消耗量、旷场实验的水平运动评分以及垂直运动评分差异均有统计学意义(F=4.091、7.670、11.008和30.316,P<0.05);与模型组和伪刺激组相比,高频组和低频组大鼠体重减分率、蔗糖水消耗量、旷场实验的水平运动评分以及垂直运动评分均升高(P<0.05),且高频组与低频组相比,差异均无统计学意义(P>0.05)。结论:rTMS干预可以改善慢性应激抑郁模型大鼠的抑郁样行为;高频rTMS和低频rTMS对大鼠抑郁行为的改善作用基本相同。

Aim： To explore the behavioral changes of chronic stress depression model rats given different frequency repetitive transcranial magnetic stimulation（rTMS） intervention. Methods： Forty adult male SD rats were allocated into normal control group（n = 8） and model preparation group（n = 32） randomly after screening. The rats in model preparation group were singly housed and given chronic unpredictable mild stress（CUMS） to build depression model. The rats in model preparation group were then allocated into 4 groups： model group（n = 8,without giving any treatment）, high frequency group（n = 8,with the intervention of 10 Hz rTMS）, low frequency group（n = 8,with the intervention of 1 Hz rTMS） and sham group（n = 8,simulation of rTMS environment without rTMS stimulus）. The changes of behaviors in each group were detected by weight measurement, sucrose consumption test and open-field test. Results： There were significant differences in the weight reduction rate, sucrose consumption and the score of open field test among the 5 groups（F = 4. 091,7. 670, 11. 008, and 30. 316,P 〈 0. 05）;compared with model group and sham group, the weight reduction rate, sucrose consump-tion and the score of open field test in high frequency group and low frequency group were higher（P 〈 0. 05）,and the differ-ence between high frequency group and low frequency group was not significant（P 〉 0. 05）. Conclusion： rTMS could im-prove the depression-like behavior in chronic stress depression model rats, and the improvement is basically identical be-tween high frequency rTMS stimulation and low frequency rTMS stimulation.