摘要
以日本优质粳稻关东194为条纹叶枯病抗性基因Stv-bi和低直链淀粉含量基因Wx-mq的供体,采用回交育种的策略,改良武育粳3号的抗性和食味品质。在连续回交和自交的过程中,利用与Stv-bi和Wx-mq基因相关的分子标记进行跟踪检测,并结合条纹叶枯病的田间抗性鉴定以及农艺性状的系统选择,在BC3F4世代中筛选到10个具有Stv-bi Stv-bi WxmqWx-mq纯合基因型的改良系。性状分析表明,这些品系保留了受体亲本武育粳3号农艺性状,条纹叶枯病抗性得到明显改良,同时,食味、外观品质也大幅提升。综合分析,最终筛选出K01和K04两个优异品系。
The japonica rice variety Kantou 194 was used as the donor of the resistance gene Stv-bi and the low-amylose content gene Wx-mq to improve the resistance and eating quality of Wuyujing 3by backcross.In continuous backcross and selfcross,molecular markers linked with Stv-b^i and Wx-mq were utilized for genotypic detection in combination with resistance identification to rice stripe disease and agronomic trait selection.Finally,10 lines with homozygous genotype Stv-b^i Stv-b^i Wx-mqWx-mq were obtained from BC3 F4 generation.The results of comparative analysis indicated that with similar agronomic traits to the receptor parent Wuyujing 3,those lines had substantial improments in resistance,appearance and eating quality.By comprehensive evaluation,two excellent lines K01 and K04 were selected.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2015年第5期467-474,共8页
Chinese Journal of Rice Science
基金
现代农业产业技术体系建设专项基金资助项目(CARS-01-47)
江苏省农业科技自主创新资金项目(CX[12]1003)
江苏省科技支撑计划资助项目(BE2012303)