聚合Stv-b^i和Wx-mq基因改良武育粳3号条纹叶枯病抗性和食味品质

Improving Resistance to Rice Stripe Disease and Eating Quality of Wuyujing 3 by Pyramiding Stv-b^i and Wx-mq

以日本优质粳稻关东194为条纹叶枯病抗性基因Stv-bi和低直链淀粉含量基因Wx-mq的供体,采用回交育种的策略,改良武育粳3号的抗性和食味品质。在连续回交和自交的过程中,利用与Stv-bi和Wx-mq基因相关的分子标记进行跟踪检测,并结合条纹叶枯病的田间抗性鉴定以及农艺性状的系统选择,在BC3F4世代中筛选到10个具有Stv-bi Stv-bi WxmqWx-mq纯合基因型的改良系。性状分析表明,这些品系保留了受体亲本武育粳3号农艺性状,条纹叶枯病抗性得到明显改良,同时,食味、外观品质也大幅提升。综合分析,最终筛选出K01和K04两个优异品系。

The japonica rice variety Kantou 194 was used as the donor of the resistance gene Stv-bi and the low-amylose content gene Wx-mq to improve the resistance and eating quality of Wuyujing 3by backcross.In continuous backcross and selfcross,molecular markers linked with Stv-b^i and Wx-mq were utilized for genotypic detection in combination with resistance identification to rice stripe disease and agronomic trait selection.Finally,10 lines with homozygous genotype Stv-b^i Stv-b^i Wx-mqWx-mq were obtained from BC3 F4 generation.The results of comparative analysis indicated that with similar agronomic traits to the receptor parent Wuyujing 3,those lines had substantial improments in resistance,appearance and eating quality.By comprehensive evaluation,two excellent lines K01 and K04 were selected.