白酱感冒颗粒提取工艺及质量研究

Extraction process and quality research of Baijiang Ganmao Granule

目的：研究白酱感冒颗粒的提取工艺及其质量标准。方法：选用L9（34）进行正交设计实验,对白酱感冒颗粒的提取工艺进行筛选,以东莨菪内酯含量为指标,考察提取溶剂、提取时间、热回流速度、溶剂倍数4个因素;采用HPLC法测定东莨菪内酯的含量,色谱柱为依利特C18色谱柱（4.6mm×250mm,5μm）;流动相为乙腈-水-冰醋酸（15∶85∶0.4）;检测波长为343nm。结果：采用热回流提取技术加水煎煮2次,第一次提取2h,第二次提取1h,每次加水15倍,热回流速度为1.5L/h（以每kg药材计）提取效果最好;东莨菪内酯在2~12μg/m L线性关系良好（r=0.9998）,平均回收率100.5%,RSD为1.69%。结论：该方法稳定可靠,适合工业化大生产。

Objective： To study the extraction process and quality standard of the Baijiang Ganmao Granule. Methods： Making an L9（ 34） orthogonal experimental design,with the content of Scopoletin as the evaluation index and with the extraction solvent,extraction time,heat reflux rate,solvent multiples as the four factors. Finding out a optimization of the Baijiang Ganmao Granule extraction process; Using HPLC determination of Scopoletin,the column was Elite C18column（ 4. 6mm × 250 mm,5μm）; mobile phase of acetonitrile- water- acetic acid（ 15∶ 85 ∶ 0. 4）; detection wavelength of 343 nm. Results： Using the heat reflux extraction technology boil water 2 times,the first extract 2 hours and the second 1 hour,added 15 times of water every time,Heat reflux rate 1. 5L / h（ per kg herbs） extracts the best; good linear indications showed as a results of the scopoletin being controlled within 2 ~ 12μg / m L（ r =0. 9998）,the average recovery was 100. 5%,RSD is 1. 69%. Conclusion： The method was stable and reliable,suitable for industrial production.