六味地黄汤辅助环磷酰胺对荷H22小鼠肝癌组织VEGF及VEGFR2蛋白表达的影响

Effect of Liuwei Dihuang Decoction for Assisting Cyclophosphamide on VEGF,VEGFR2 Expression in H22 Tumor-bearing Rats

目的:观察六味地黄汤辅助环磷酰胺对荷H22肝癌小鼠肝癌组织VEGF及VEGFR2蛋白表达的影响。方法:雄性昆明小鼠40只,随机分为模型组、化疗组、六味组、六味化疗联合组,每组10只。腋下注射H22细胞建立H22荷瘤模型,六味组造模前两周开始用药[剂量为22 g/(kg·d)],化疗组[剂量为50mg/(kg·d),隔日1次]及六味化疗组造模后用药,造模14 d后处死小鼠称重,取瘤组织称重计算抑瘤率,取脾组织称重计算脾指数,免疫组化检测VEGF与VEGFR2蛋白的表达。结果:化疗组与六味化疗联合组有良好的抑瘤作用;六味组脾指数增大,与模型组比较差异具有统计学意义(P<0.05),六味化疗联合组VEGF蛋白表达明显减少,与化疗组比较,差异有统计学意义(P<0.05),与六味组相比较,差异有统计学意义(P<0.05);六味化疗联合组VEGFR2蛋白表达减少,与模型组比较,差异有统计学意义(P<0.05);与六味组比较,有显著性差异(P<0.01)。结论:六味地黄汤辅助环磷酰胺可抑制荷H22肝癌小鼠组织VEGF与VEGFR2蛋白表达,减少肝癌组织血管增殖,从而发挥辅助化疗作用。

Objective： To observe the effect of Liuwei Dihuang decoction（ LW） for assisting cyclophosphamide（ CTX） on VEGF,VEGFR2 expression in H22 tumor- bearing mice. Methods： 40 male Kunming mice were randomly divided into model group,CTX group,LW group,the LW combined CTX group,10 rats in each group. The establishment of H22 tumor model by axillary injection of H22 cells,LW group began to use medication of two weeks before molding [dose of 22 g /（ kg·d） ],CTX group [at a dose of 50 mg /（ kg·d）,every other day for 1 time]and LW group for drug after molding. Put the mice to death after 14 days of the group establishment,then take weighing,take tumor tissue weighing to calculate tumor- inhibition rate,spleen tissue weighing to calculate the spleen index,make the protein expression after immunohistochemical detection of VEGF,VEGFR2. Results： the CTX group and combination group had a significant role in inhibiting tumor; LW group spleen index increased,the difference being statistically significant compared to the model group（P〈0. 05）,combination group＇s VEGF protein expression decreased obviously,compared with CTX group,the difference was statistically significant（P〈0. 05）,compared with LW group,the difference was statistically significant（P〈0. 05）; combination group＇s VEGFR2 protein expression decreased obviously,compared with the model group,there was statistical significance difference（P〈0. 05）; compared with LW group,there was significant difference（P〈0. 01）. Conclusion： The LW could enhance the antitumor effects of CTX. Its mechanism may be related to the combination which can reach the synergistic inhibitory effects of VEGF and VEGFR2 protein expression.