基于能量代谢技术对高脂饲料干预的小鼠脂代谢研究

The lipid metabolism based on the energy metabolism technology in obese mice induced by a high-fat diet

目的研究长期高脂饲料对机体能量及脂肪代谢的影响。方法 20只雄性清洁级C57BL/6小鼠,随机分为对照组和高脂组,分别喂饲基础饲料和高脂饲料。第12周末,间接测热法检测小鼠的总能量消耗及物质代谢情况。多排定量CT扫描仪检测小鼠全身脂肪含量和分布。实验结束处死小鼠,留取血样和肝脏组织,进行生化指标及组织病理学检测。结果与对照组比较,高脂组小鼠能量摄入增多,全身和内脏脂肪含量增加,脂肪酸氧化水平显著升高(P<0.05);空腹血糖、血脂、胰岛素以及肝脏和血清中的脂肪酸含量皆显著高于对照组(P<0.05);肝细胞脂肪变性,线粒体受损。结论长期高脂饲料能导致机体能量摄入过多,进而导致脂肪代谢紊乱,出现明显腹部肥胖、高脂血症、脂肪肝和胰岛素抵抗。

Objective To ingvestigate effects of long-term high-fat diet on the energy and lipid metabolism. Methods 20 SPF male C57 BL /6 mice were randomly divided into a control group fed a basic diet and a high-fat group fed a high-fat diet. Indirect calorimetry measurement was done to mearue total energy expenditure and metabolism in the mice at the 12 th week. Multi-row quantitative CT was applied to detect body fat content and distribution in the mice. At the end of the experiment the mice were killed,and blood and liver were collected to detect the biochemical and histopathological indicators. Results In comparison with the control group,the mice fed high-fat diet increased energy intake,body and visceral fat content and levels of fatty acid oxidation were significantlyincreased（ P〈0. 05）. Fasting blood glucose,blood lipid,insulin and fatty acid content in liver and serum were significantly higher than those in the control group（ P〈0. 05）. Pathology examinations showed hepatocytes steatosis,mitochondria and other organelles structural damage in the high-fat mice. Conclusion Long-term high-fat diet can lead to excessive energy intake, lipid dysmetabolism, abdominal obesity,hyperlipidemia,fatty liver and insulin resistance.