微波辐射对家兔心脏损伤及其机制的研究

The cardiac injury effect of microwave radiation on rabbit and its mechanism

目的探讨不同强度微波辐射对家兔心脏的损伤作用及其机制。方法分别以强度为50、100、150和200 m W/cm2,频率为2450 MHz微波照射家兔20 min,照射后6 h,取家兔心脏,检测心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性,HE染色观察心脏组织结构的改变,Western blotting检测心肌细胞内Caspase-3和HSP 70蛋白表达量变化。结果随着微波辐射强度的增加(100、150和200 m W/cm2),辐射组家兔心肌细胞中ATP含量、线粒体呼吸链复合体Ⅳ和Ⅴ活性呈现降低趋势,与对照组相比,差异具有统计学意义(P<0.05)。HE染色结果显示,微波辐射组家兔心肌细胞轻度水肿,肌纤维排列不整,心肌细胞出现明显的损伤。Western blotting结果显示,微波辐射后心肌细胞内Caspase-3和HSP 70蛋白表达水平随着辐射强度的增加呈上升趋势(P<0.05)。结论微波辐射对家兔心脏具有明显的损伤作用,其可能机制是通过改变心肌细胞内应激水平而引发细胞凋亡。

Objective To investigate the cardiac injury effect of different intensities microwave radiation on rabbits and its possible mechanism. Methods Rabbits were radiated by intensity of 50,100,150 and 200 mW / cm^2 2450 MHz microwave for 20 min.6 h after microwave radiation,the heart tissue was taken. ATP and mitochondria complexⅣ and Ⅴ were measured in myocardial cells. The changes of myocardial tissue were observed by light microscopic. The expression of Caspase-3 and HSP 70 were detected by western blotting. Results The activity of ATP and mitochondria complex Ⅳ and Ⅴdecreased significantly compared with normal control in cardiac tissue. 100、150 and 200 mW / cm^2 microwave radiation group vs. control group（ P〈0. 05）. The HE staining result showed that myocardial cell appears edema,muscle fiber malalignment,cells appeared obvious injury. Results of western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group（ P〈0. 05）.Conclusion Microwave radiation has injury effect on rabbit heart. The possible mechanism may be related with inducing cell apoptosis by changing of stress level inmyocardial cell.