连翘苷元对大鼠免疫性肝纤维化的影响

Effect of forsythiaside on immunological hepatic fibrosis of rats

目的研究连翘苷元对牛血清白蛋白诱导的大鼠免疫性肝纤维化的影响。方法动物按照体质量随机分为对照组、模型组、连翘苷元低、中、高剂量(0.1、0.3、1.0 mg/kg)组和水飞蓟宾葡甲胺组(50 mg/kg),利用牛血清白蛋白建立大鼠免疫性肝纤维化模型。全自动生化分析仪检测血清蛋白水平;Elisa试剂盒检测血清透明质酸(HA)、层黏蛋白(LN)、Ⅳ型胶原(Ⅳ-C)和Ⅲ型前胶原(PCⅢ)水平;分光光度计法检测肝脏组织羟脯氨酸(Hyp);取肝左叶固定、HE染色,显微镜下观察肝纤维化程度。结果连翘苷元能够显著降低肝纤维化大鼠血清HA、LN、Ⅳ-C和PCⅢ的量,减少肝脏质量和肝脏系数,降低肝脏Hyp水平,减轻肝纤维化程度。结论连翘苷元对免疫性大鼠肝纤维化有较好的治疗作用。

Objective To investigate the effects of forsythiaside on immunological hepatic fibrosis induced by bovine serum albumin （BSA） in rats. Methods To establish the immunological hepatic fibrosis models induced by BSA. Animals were randomly divided into six groups namely normal group, model group, forsythiaside （0.1, 0.3, and 1.0 mg/kg） treated groups, and Silybin group （50 mg/kg）. The levels ofTP, ALB, AST, and ALT were assayed by automatic biochemical analyzer; The levels of LN, HA, IV-C and PCⅢ in serum were assayed by ELISA kit; Hydroxyproline （Hyp） content in liver tissue was assayed by spectrophotometry; Taking the left hepatic lobe fixed, HE staining was used to examine the histopathological change under microscope. Results Forsythiaside could significantly alleviate the hepatic fibrosis, not only in decreasing the Hyp content in hepatic tissue, but also reducing the levels of LN, HA, Ⅳ-C and PCⅢ in rats with immunological hepatic fibrosis. Conclusion Forsythiaside has the protective effects on the hepatic fibrosis induced by BSA in rats.