内皮祖细胞对脂多糖刺激活化后中性粒细胞分泌功能的影响

The secretion function of polymorphonuclear cells after lipopolysaccharide stimulation when co - cultured with endothelial progenitor cells

目的观察内皮祖细胞（EPC）对脂多糖刺激大鼠中性粒细胞（PMN）生成肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、中性粒细胞蛋白酶（NE）及基质金属蛋白酶-9（MMP-9）的影响。方法分别分离培养EPC及PMN，随机分为3组（PBS组、LPS组和EPC组），EPC组以1×l矿／孔密度将EPC接种于24孔板，贴壁后再加入同等密度的PMN，用终浓度1μg／mL的脂多糖刺激活化6h；PBS组及LPS组中仅加入同等密度的PMN，分别给予等浓度的PBS及LPS刺激6h；分别收集培养的上清及细胞检测各组TNF—α、IL-1β、NE及MMP-9的表达。结果LPS组中TNF—α、IL-1β、NE及MMP-9表达较PBS组显著增加，而它们在EPC组的表达较LPS组显著降低（P〈0．05）。结论EPC能抑制PMN的分泌功能。

Objective To explore the influence of endothelial progenitor cells （EPC） on polymorphonuclear ceUs （PMN） secretory factors stimulated by lipopolysaccharide （LPS）. Methods EPC and PMN were separated and cultured respectively, and they were divided into three groups. EPC were incubated in a 24 - well plate at a density of 1 × 105 per well. After adherence, PMN with the same density were added in the medium, and 1 μg/mL LPS was applied to the mixture for 6 hours. There are same density PMN in PBS and LPS groups stimulated by equidensity PBS and LPS respectively. Supematant and cells were collected for determination, and the changes of tumor necrosis factor - α （ TNF - α）, interleukin - 1β （ IL - 1β）, neutrophil elastase （NE） and matrix metallopmteinases - 9 （ MMP - 9） in three groups were detected. Results TNF - α, IL - 1β, NE and MMP - 9 contents were remarkably increased with stimulated by LPS compared with PBS group （ P 〈 0. 05 ）. When EPC were added, all productions significantly reduced （P 〈 0. 05 ）. Conclusion EPC can inhabit the activation of PMN after stimulating with LPS.