类风湿性关节炎患者CXC趋化因子配体16表达及临床意义

The expression of CXCL16 and its clinical significance in patients with rheumatoid arthritis

目的探讨类风湿关节炎(RA)患者血清CXC趋化因子配体16(CXCL16)水平变化及临床意义。方法采用酶联免疫吸附法(ELISA)检测200例RA患者(RA组)、180例骨关节炎患者(OA组)及200例健康体检者(正常对照组)血清CXCL16水平,比较组间CXCL16水平差异;根据关节疾病活动度评分(DAS28),将RA组分为高度活动组、中度活动组和低度活动组,比较组间CXCL16水平差异,并分析DAS28评分与CXCL16水平的相关性。结果 RA组血清CXCL16水平明显高于OA组和正常对照组,差异有统计学意义(P<0.05);OA组与正常对照组之间CXCL16水平差异无统计学意义(P>0.05);RA高度活动组、中度活动组和低度活动组之间CXCL16水平差异有统计学意义(P<0.05)。各组CXCL16水平与DAS28评分均呈正相关(r值分别为0.820、0.674、0.748,P<0.01)。结论血清CXCL16可作为RA的实验室诊断指标,也可作为病情观察和预后判断的依据。

Objective To discuss the changing levels of serum CXC chemokine ligand 16（ CXCL16） in patients with rheumatoid arthritis（ RA） and its clinical significance. Methods Using enzyme- linked immunosorbent assay（ ELISA）,we measured the concentration of serum CXCL16 in 200 patients with RA（ RA group）,180 patients with osteoarthritis patients（ OA group）and 200 healthy persons（ normal control group）,their different levels of CXCL16 were compared among the groups. According to the joint disease activity score（ DAS28）,RA group was divided into the high activity group,moderate activity group and low activity group,the different CXCL16 levels were compared among these groups,and we analyzed the correlation between DAS28 score and CXCL16 level. Results The level of serum CXCL16 in RA group was significantly higher than that in OA group and normal control group,and the difference had statistical significance（ P〈0. 05）; the level difference of CXCL16 between OA group and normal control group was not statistically significant（ P〈0. 05）. In RA group,there was no statistical significance on the difference of CXCL16 levels of high activity group,moderate activity group and low activity group（ P〈0. 05）. CXCL16 level and DAS28 score were positively correlated in each group（ r = 0. 820,0. 674,0. 748,P〈0. 01）. Conclusion Serum CXCL16 can be used as laboratory index in the diagnosis of RA,which can also be used as the observation and prognosis judgment basis.