理冲汤调控人子宫肌瘤细胞MMP-2、TIMP-2表达的研究

Study on Lichong Decoction on the Expression of MMP-2 and TIMP-2 in Human Uterine Leiomyoma Cells

目的研究理冲汤调控人子宫肌瘤细胞内基质金属蛋白酶-2(MMP-2)及其抑制物(TIMP-2)的表达。方法进行人子宫肌瘤细胞原代、传代培养及鉴定后,加入理冲汤含药动物血清干预,电镜观察人子宫肌瘤细胞微观结构的改变,蛋白质印迹(Western Blotting)技术观察MMP-2以及TIMP-2在人子宫肌瘤细胞内的含量与表达。结果电镜显示,与空白对照组比较,理冲汤组细胞排列基本正常,细胞器基本接近正常,线粒体增生肥大减轻,胶原纤维排列基本规则,增生不明显;Western Blotting显示,与空白对照组比较,理冲汤组的MMP-2表达显著降低(P<0.05,P<0.01),而TIMP-2的表达显著升高(P<0.05)。结论理冲汤具有抑制体外人子宫肌瘤细胞生长的作用,其机制可能与降低人子宫肌瘤细胞中MMP-2的表达,以及提高人子宫肌瘤细胞中TIMP-2的表达有关。

Objective To investigate Lichong Decoction on the expression of Matrix metalloprotein-ase - 2（MMP - 2）and Tissue inhibitors of metalloproteinase - 2（ TIMP - 2）in human uterine leiomyoma cells. Methods The primary culture and subculture of the human uterine leiomyoma cells were performed and identified. The cells were treated with Lichong Decoction. Electron microscope was used to observe micro-structure changes in the uterine leiomyoma cells. Western blotting was used to measure the expression of MMP - 2 and TIMP - 2 in human uterine leiomyoma cells. Results The transmission electron microscope pathological examination showed cells of Li Chong Decoction group arranged basically normal,cellular organs were quite close to the normal,hyperplasia and hypertrophy of chondriosome were reduced,collagen fibers ar-ranged basically regularly,hyperplasia was not obvious. Western blotting showed the expression of MMP - 2 significantly was reduced in Lichong Decoction group（P ﹤ 0. 05,P ﹤ 0. 01）. However,the expression of TIMP- 2 significantly increased in this group（P ﹤ 0. 05）. Conclusion Lichong Decoction can inhibit the growth of uterine leiomyoma cells. Its mechanism may be related to reducing the expression of MMP - 2,as well as improving the expression of TIMP - 2 in uterine leiomyoma cells.