miRNA-145抑制肺癌细胞系A549迁移侵袭的研究

Inhibitory Effect of Micro RNA-145 on Migration and Invasion of Human Lung Cancer Cell Line A549

[目的]观察mi RNA-145对肺癌细胞系A549迁移、侵袭和克隆形成的影响,探讨其潜在的应用价值。[方法]采用mi RNA-145过表达的慢病毒载体上调A549细胞中mi RNA-145的表达量,Transwell迁移及侵袭实验分析mi RNA-145表达上调前后A549细胞的体外迁移、侵袭能力,平板克隆形成实验分析mi RNA-145表达上调前后A549细胞的体外增殖能力。[结果]RT-PCR结果表明,侵染了mi RNA-145过表达载体的A549细胞中mi RNA-145水平明显高于阴性对照组及空白对照组,差异有统计学意义(P<0.01);Transwell迁移和侵袭实验结果表明,过表达mi RNA-145的A549细胞过膜数少于阴性对照及空白对照组,差异有统计学意义(P<0.01);平板克隆形成实验结果表明,过表达mi RNA-145的A549细胞平板克隆形成能力明显降低,差异有统计学意义(P<0.01)。[结论]mi RNA-145的过表达能够抑制肺癌细胞系A549的迁移、侵袭和克隆形成,由此提示mi RNA-145有可能成为肺癌治疗的潜在靶点。

[Purpose] To observe the effect of mi RNA-145 on the migration and invasion and proliferation abilities of human lung cancer cell line A549,and to discuss its potential application.[Methods] Hsa-mi R-145-5p was used to increase mi RNA-145 expression in A549 cell lines,then the migration ability of A549 cell was observed by Transwell migration assay before and after treatment with hsa-mi R-145-5p,the invasive abilitiy of A549 cell was observed by Transwell invasion assay before and after treatment with hsa-mi R-145-5p,the proliferation ability of A549 cell was observed by plate clone formation assay before and after treatment with hsa-mi R-145-5p.[Results] The result of real-time RT-PCR showed that mi RNA-145 expression in A549 cell infected by hsa-mi R-145-5p was higher than others（P〈0.01）. Up-expression of mi RNA-145 in A549 cell inhibited the migration and invasion and proliferation abilities of A549 cells（P〈0.01）. [Conclusion] Up-expression of mi RNA-145 plays an inhibitory role in the migration and invasion and proliferation of human lung cancer cell line A549,which indicate that it might be a new target for biotherapy of lung cancer.