鸢尾素通过抑制氧化应激减轻高糖/高脂诱导的人脐静脉内皮细胞损伤

Irisin exerts protective effects against high-glucose / high-fat-induced injury of human umbilical vein endothelial cells through alleviating oxidative stress levels

目的研究鸢尾素(irisin)是否通过抑制氧化应激减轻高糖/高脂所致心肌细胞损伤。方法将培养的人脐静脉内皮细胞(Human umbilical vein endothelial cells,HUVECs)分为3组:正常对照组、高糖/高脂组及高糖/高脂+irisin组。各组培养24 h后检测细胞存活率、凋亡率、ROS产量、NADPH氧化酶gp91phox表达水平。结果与正常对照组相比,高糖/高脂组细胞存活率显著下降,ROS产量、NADPH氧化酶gp91phox表达水平、细胞凋亡率显著升高(均P<0.05),irisin处理可以逆转上述改变(均P<0.05)。结论高糖/高脂能够增加人脐静脉内皮细胞氧化应激,促进细胞凋亡;irisin可通过减轻氧化应激而发挥内皮保护作用。

AIM To investigate whether irisin exerts endothelial protection in response to high-glucose/ high-fat damage through reducing reactive oxygen species （ROS） formation. METHODS Cultured human umbilical vein endothelial cells （HUVECs） were randomly divided into control group （5 mmol/L glucose and 20 mmol/L mannitol）, high-glucose/high-fat group （25 mmol/L glucose and 500 /xmol/L sodium palmitate, 24 h incubation） and high-glucose/high-fat ＋ irisin group （25 mmol/L glucose, 500 p, moL/L sodium palmitate and 1 pLg,/ml irisin, 24 h incubation）. Cells from these groups were harvested for evaluation of cell viability, ROS formation, NADPH oxidase gpgl pho~ expression and apoptosis rate. RESULTS Compared with those in the control group, cell v/ability decreased, whereas gp91 p~~~ expres- sion, ROS formation and apoptosis rate increased in high-glucose/high-fat group （ all P 〈 0. 05 ）. These changes were all reversed by irisin （ all P 〈 0. 05 ）. CONCLUSION High-glucose/high-fat leads to oxidative stress in HUVECs and Jr/sin can protect HUVECs by alleviating oxidative stress and endothelial apoptosis.