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孕期母血清miRNA-19b诊断胎儿先天性心脏病的初步评价 被引量：1

Clinical value of prenatal maternal serum miRNA-19b in diagnosis of fetal congenital heart disease
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摘要 目的探讨孕期母血清miRNA在胎儿先天性心脏病（CHD）诊断中的临床价值。方法选取2009年7月-12月27例我院孕中期CHD胎儿孕妇为研究对象，同时收集27例与病例组在孕妇年龄、胎儿孕周基本匹配的胎儿无畸形的孕妇作为对照组，采用Real—timePCR检测miRNA-19b的表达水平，采用ROC曲线下面积（are aunder the curve，AUC），对miRNA-19b诊断CHD胎儿的准确性进行初步评价。结果通过检测孕期母血清miRNA-19b的表达水平，结果表明胎儿CHD病例组孕妇血清miRNA-19b水平显著高于对照组（P〈0．01），对miRNA-19b预测胎儿CHD的准确性进行分析，结果表明AUC分别为0．79。结论孕期母血清miRNA-19b可以预测胎儿CHD的发生风险，作为胎儿CHD的诊断标志物。 AIM To investigate the clinical value of prenatal maternal serum miRNA-19b in the diagnosis of fetal congenital heart disease （CHD）. METHODS Twenty-seven pregnant women with fetal CHD in our hospital between July 2011 and June 2012 were included in the study group and 27 pregnant women without fetal CHD were selected as control group. We performed real-time PCR assays to identify and validate the expression of maternal serum miRNAs in fetal CHD. Area under the curve （AUC） was used to evaluate the diagnostic accuracy of the maternal serum miRNA-19b for fetal CHD. RESULTS miRNA- 19b was significantly upregulated in pregnant women with fetal CHD, which was validated by real-time PCR. AUC for diagnostic accuracy of miRNA-19b was 0. 79. CONCLUSION Maternal serum miRNAs （miRNA-19b） could act as novel and non-invasive biomarkers for prenatal detection of fetal CHD.
作者 沙莉 朱莎莎 余章斌 韩树萍
机构地区 南京医科大学附属南京妇幼保健院儿科
出处 《心脏杂志》 CAS 2015年第5期609-612,共4页 Chinese Heart Journal
基金 国家自然科学基金项目资助(81070500) 南京市医学科技发展资金项目资助(QRX11107)
关键词 心脏病 先天性 miRNA-19b 诊断标志物 congenital heart disease miRNA-19b diagnostic marker
分类号 R541.1 [医药卫生—心血管疾病]
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参考文献18

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二级参考文献21

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心脏杂志
2015年 第5期

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