免疫荧光灶试验法检测消毒剂灭活病毒效果的方法研究

Research on virus inactivation effect detection of disinfectant by fluorescent focus assay

目的建立一种检测消毒剂灭活病毒效果的方法。方法采用免疫荧光灶试验方法,建立消毒剂灭活病毒效果的检测方法。结果本研究建立的荧光灶试验法实验证明,采用浓度为2 000 mg/L季铵盐复合消毒剂对悬液内狂犬病毒作用3 min,平均灭活对数值大于4.0。实验浓度证明该消毒液对病毒宿主细胞生长有影响,但本研究选用的中和剂可有效中和最高实验浓度的消毒剂对病毒及其宿主细胞的影响。结论本研究建立的免疫荧光灶试验方法,可以用于该皮肤黏膜消毒剂灭活狂犬病毒效果的检测,操作简便,出结果快,利于统计计算,便于观察。

Objective To establish a method for virus inactivation detection of disinfectant. Methods Immunofluores- cence method was used to establish of the disinfectant effect of virus inactivation. Results The fluorescent focus assay method experiment showed that the rabies virus was inactivated within 3 min by 2000 mg/L quaternary ammonium com- pound disinfectant in suspension, the average logarithm value was greater than 4. The experiments showed that the disin- fectant affected the normal cell growth, but the neutralizing agent could effectively neutralize the highest concentration of the disinfectant on the virus and its host cell. Conclusion Immune fluorescent focus assay established in this study can be used to detect of skin and mucosa disinfectant inactivating virus effect, it is simple operation, results quick, convenient for observation.