摘要
目的：建立癫痫患者血浆中丙戊酸钠（VPA）代谢产物2-丙基-2-戊烯酸（2-ene-VPA）的HPLC测定方法。方法：血浆用乙酸乙酯提取,以2,4’-二溴苯乙酮作为内标,氮气挥干样品,色谱柱：ZORBAX SB-C18柱（4.6 mm×150 mm,5μm）;流动相：甲醇-水（55∶45）;检测波长：217 nm;流速：1.0ml·min^-1;柱温：35℃;进样温度：20℃。结果：内标2,4’-二溴苯乙酮和2-ene-VPA的保留时间分别为11.2 min和5.1 min,2-ene-VPA的血浆浓度在0.5-16.0μg·ml^-1范围内,2-ene-VPA与内标2,4’-二溴苯乙酮的峰面积比与浓度呈良好的线性关系（R〉0.999）,平均回收率为（96.85±0.33）%,日内RSD〈5%,日间RSD〈5%。结论：本法经济、快速、简便,适用于临床上2-ene-VPA的测定及研究。
OBJECTIVE To establish a rapid analysis method for determination of sodium valproate metabolite 2-ene-VPA in plasma by RP-HPLC.METHODS Plasma sample was extracted with ethyl acetate,2,4＇-dibromoacetophenone as an internal standard.Nitrogen gas was used to dry samples under the following conditions：UV detector（217 nm）and ZORBAX SB-C18column（150 mm×4.6 mm,5μm）;mobile phase consisted of methanol-water（55∶45）;flow rate 1.0ml·min^-1;column temperature 35 ℃.RESULTS Retention times of 2-ene-VPA and internal standard were 5.1 min and 11.2 min,respectively.Linear range was 0.5-16μg·ml^-1.Average recovery rate was（96.85±0.33）%.Inter-day and intra-day relative standard deviation（RSD）was all less than 5%.CONCLUSION This method is economic,rapid,convenient and suitable for clinical routine monitoring and research of 2-ene-VPA.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2015年第18期1654-1658,共5页
Chinese Journal of Hospital Pharmacy
基金
贵州省卫生厅基金(编号:gzwkj2010-1-003)
