离子注入一串红的生物学效应研究

Study of Biological Effect of Ion Implantation on Salvia splendens

本文利用离子注入技术,将不同能量、不同剂量的铁离子和铝离子分别注入一串红种子,通过分析萌发率、株高、冠幅、花序长、叶绿素相对含量等指标,探讨不同离子注入处理对一串红生物学效应的影响。结果表明,处理对一串红种子萌发率表现出抑制作用,其中高能量铝离子注入对叶色加深有促进作用;各处理对一串红株高、冠幅、花序长和叶绿素相对含量的影响差异极显著,其中Ⅰ号(70ke V-Fe3+-5×1015ions·cm-2)株高均值最小,为15.67cm;Ⅶ号(4×103ke V-AL3+-6×1012ions·cm-2)冠幅均值最大,为22.97cm;Ⅷ号(4×104ke V-AL3+-6×1011ions·cm-2)花序长和叶绿素相对含量均值最高分别为10.32cm和44.68。本研究得到了不同指标利于提高观赏性的剂量值,预示着离子注入技术在一串红育种中具有广阔的应用前景。

Using the technology of ion implantation, the Fe3＋ ions and Al3＋ ions with different energy and dose were implanted to Salvia splendens seeds. By analyzing the indexes including germination rate, plant height, crown width, verticillaster length and chlorophyll relative content, the influence of different ion implantation treatments on biological effect of Salvia splendens were discussed. The results revealed that the eight treatments inhibited the germination rate of Salvia splendens seeds, while high engery AL3＋ ion implantation caused accelerative effect on the leaf colour. Plant height, the crown width, verticillaster length and chlorophyll relative content of Salvia splendens were markedly affected by the nine treatments. Among these treatments, the minimum average plant height of I treatment （70keV -Fe3＋ - 5 ×10^15ions· cm 2） was 15.67cm, the maximum average crown width of VII treatment（4×103keV - AL3＋ - 6×10^12 ions·cm-2）was 22.97cm, the highest average verticillaster length and chlorophyll relative content of VM treatment （4MeV - AL3＋- 6 ×10^11 ions· cm-2） were 10.32cm and 44.68 respectively. The doses having for different indexes were obtained. The results indicated that the technology of ion implantation has widely prospect on the application of breeding in Salvia splendens.