黑果枸杞对辐射损伤小鼠造血系统的保护作用

Protective Effect of Lycium ruthenicum on Peripheral Blood System Against Radiation Injury in Mice

目的:探讨黑果枸杞对辐射损伤小鼠造血系统的防护作用。方法:昆明种小鼠分为正常对照组、辐射模型组、氨磷汀组及黑果枸杞高(8 g/kg)、中(4 g/kg)、低(2 g/kg)剂量组,于辐射后第3天进行实验,同法分组分别于辐射后第7、14天进行实验。给药组灌胃给予黑果枸杞水提取液,正常对照组与模型组给予等体生理盐水,连续14 d,氨磷汀组于辐射前30 min腹腔注射氨磷汀。除正常对照组外各组小鼠接受X射线一次性全身照射。辐射后第3、7、14天分别观察各组小鼠血象、脏器指数、骨髓DNA含量、血清Caspase-3、Caspase-6含量及小肠隐窝上皮细胞P53含量变化情况。结果:黑果枸杞能升高辐射后小鼠红细胞计数和血红蛋白含量(P<0.05),升高小鼠胸腺指数及脾脏指数(P<0.05),显著提高辐射小鼠骨髓DNA含量(P<0.05),降低小鼠血清Caspase-3、Caspase-6含量及小肠隐窝上皮细胞P53蛋白的表达。结论:黑果枸杞水提取液对辐射损伤小鼠造血系统有一定保护作用。

Objective：To study the protective effect of Lycium ruthenicum on peripheral blood system against radiation injury in mice. Methods：Kunming mice were randomly divided into control group, model group, positive group and Lycium ruthenicum high dose （8 g/kg） , middle dose （4 g/kg）and low dose （2 g/kg）treatment groups that experimented three days after irradiation. In the same way, groups were set at 7 days and 14 days after irradiation respectively. Lycium ruthenicum extract were administered orally to the mice in the three Lycium ruthenicum treatment groups and normal saline were administered orally to the mice in control group and model group for 14 days. Positive group were treated with radioprotective agent amifostine （ WR-2721 ） at 30 min before irradiation. Except control group,mice in other groups received quantity of 5 Gy X-radiation whole body evenly with one time. Hemogram, organ index, DNA, Caspase-3, Caspase-6 and P53 contents were observed at the 3rd ,Tth and 14th day after irradiation. Results ：Lycium ruthenicum signifi- cantly increased the total red blood cell count, hemoglobin count, the indexes of spleen and thymus and bone marrow DNA contents （P 〈 0.05） ,as well as decreased Caspase-3 and Caspase-6 contentsin serum and the expression of P53 in intestinal crypt epithelial cells. Conclusion：The results showed that Lycium ruthenicum had protective effects on peripheral blood system against radiation injury in mice.