玉米脱落酸受体基因家族的生物信息学分析

Bioinformatic Analysis for Abscisic Acid Perceptor Gene Family in Maize

为了研究玉米脱落酸的应答机制,综合运用相关生物信息学软件,在玉米全基因组范围内扫描鉴定玉米脱落酸受体基因家族成员,分析其染色体定位、基因结构、启动子顺式作用元件和表达模式,预测其推导蛋白的结构和功能。结果表明,玉米基因组共存在13个脱落酸受体基因Zm PYL1-13,随机分布于10条染色体上,其推导蛋白都具有脱落酸受体的保守结构域CL2和CL4。根据推导蛋白的氨基酸序列多重比对,可将这13个基因分为Ⅰ、Ⅱ、Ⅲ3个亚家族,分别包括Zm PYL9-13、Zm PYL4-8和Zm PYL1-3。第Ⅰ亚家族的5个成员分别具有2-10个内含子,第Ⅲ亚家族的Zm PYL8包含1个内含子,而第Ⅱ和第Ⅲ亚家族的其余7个成员没有内含子。在各成员的上游启动子区域,均存在激素应答、非生物逆境胁迫诱导和发育调控等相关的众多顺式作用元件。蛋白质结构预测结果表明,第Ⅰ亚家族的5个成员均为同源二聚体,而第Ⅱ和第Ⅲ亚家族的各成员,除Zm PYL8和Zm PYL3以外,都以单体形式存在。而且,玉米脱落酸受体基因家族各成员在玉米不同发育阶段和不同组织器官中的表达模式也各不相同。本研究将为玉米脱落酸受体家族的深入研究以及玉米对非生物逆境胁迫应答的分子机理解析提供依据。

To study the response mechhism of abscisic acid（ ABA） in maize. In the present study,the members of the abiscisic acid preceptor gene family were scanned genome-widely,and analyzed for their chromosome locations,gene structure,promoter cis-acting elements,expression patterns,structure and function of the putative proteins,with the integrated use of bioinformatics software. The results showed that there are thirteen members（ Zm PYL1 to 13） of abscisic acid preceptor gene family in the maize genome. They randomly distributes all over the ten chromosomes. The putative proteins contain the conserved structure domains CL2 and CL4 for abscisic acid preceptor. According to the multiple alignment among the amino acid sequences of their putative proteins,these thirteen genes were classified into three subfamilies. The subfamily Ⅰ,Ⅱ and Ⅲ include members Zm PYL9 to 13,Zm PYL4 to 8 and Zm PYL1 to 3,respectively. All the five members of subfamily I contain 2 to 10 introns,respectively,while the members of subfamilies II and III contain no intron except for Zm PYL8 of subfamily III contains 1 intron. During the upstream promoter regions of all the members,there are a number of cis-acting elements responsive to hormones,abiotic stress and development regulation. The prediction of protein structure showed that all the five members of subfamily I are homodimers,while the members of subfamilies II and III are monomers,except Zm PYL8 and Zm PYL3. On the other hand,the expression patterns of the members of the abscisic acid preceptor gene family were differential among developmental stages,and different tissues and organs. All the above results lay the foundation for further research on abscisic acid family in maize,and exploration for molecular mechanism of maize response to abiotic stress.