浙江省红颜草莓镶脉病毒(SVBV)的调查与茎尖脱毒技术研究

Investigation of Strawberry Vein Banding Virus(SVBV) in Zhejiang and Shoot Tip Detoxification Technology on Benihope Cultivar

为了解浙江省草莓主要产区红颜草莓镶脉病毒的感染情况,建立适宜的草莓脱毒快繁技术体系,调查了浙江省主要草莓产区草莓镶脉病毒的分布情况。PCR检测结果表明,大部分红颜品种的植株感染了SVBV。序列分析表明,所感染的病毒与NCBI中报道的病毒相似性达98%以上。以0.2 mm匍匐茎茎尖为外植体,用MS培养基+0.3 mg·L-16-BA+0.05 mg·L-1GA+0.01 mg·L-1IBA+3%蔗糖(p H值5.8)进行脱毒培养。PCR检测结果显示,茎尖培养植株的脱毒率达到93%。采用以上方法可以培养根系发达、抗性强的植株。试验结果将为建立完善的草莓病毒脱毒技术体系提供一定的技术指导。

In order to understand the infection of Strawberry Vein Banding Virus （SVBV） in Benihope strawberry plants in main production areas of Zhejiang, and to establish appropriate virus-free propagation system of strawberry, the distribution of SVBV in the main strawberry production regions were investigated. Results showed that most Benihope plants were infected with SVBV. The gene sequence of SVBV isolated from infected plants was compared with that of SVBV in NCBI database, that indicated that the gene of SVBV in Zhejiang province shared a high sequence similarity of 98% with that of SVBV in NCBI. Runner tips with 0.2 mm long were cultured in MS medium wih 0.3 mg·L^-16 - BA, 0.05 mg·L^-1 GA, O. 01 mg·L^-1 IBA, 3% sucrose, pH 5.8. PCR results showed that the detoxifieation rate of plant shoot tip culture was 93%. Using the above method plants with thick and strong root system and of strong resistance can be cultured. The results of this paper will provide a useful guidance for the establishment of a sound virus detoxification technique of strawberry.