不同蓝靛果忍冬品种对干旱胁迫的光合生理响应比较分析

Analysis of Comparative the Photosynthetic Characteristics and Physiological Responses of Different Varieties of Lonicera caeruleae Rehd to Drought Stress

为明确各蓝靛果忍冬品种对干旱胁迫的耐受性,釆用盆栽自然干旱法人工模拟干旱胁迫,设4个土壤干旱胁迫梯度(10%、20%、30%、40%)处理6个蓝靛果忍冬品种苗,分别为伊利亚达、北日勒、蔚蓝、巴克恰尔斯卡亚、瓦休甘斯卡亚、火红蛋白石,测定其光合及生理生态指标,系统比较各参数的适应性变化及其差异性分析。结果表明,土壤绝对含水量下降到10%时,伊利亚达净光合速率(Pn)最高,为0.68 mol·m-2·s-1,北日勒和蔚蓝的净光合速率出现负值,其余品种净光合速率也下降到最低并全部与土壤绝对含水量40%时有极显著差异(p≤0.01),伊利亚达的蒸腾速率(Tr)和水分利用率(WUE)下降较缓慢,分别比土壤绝对含水量40%时下降了67.95%、79.35%;胞间CO2浓度变化表明,非气孔因素引起了伊利亚达和巴克恰尔斯卡亚叶片的净光合速率下降。土壤绝对含水量下降到10%时,伊利亚达和北日勒叶片叶绿素含量明显高于其它品种,并且比土壤绝对含水量40%时提高了37.4%、0.62%,而可溶性糖含量缓慢上升,表明这2个品种在干旱条件下,可以通过提高叶绿素含量保证对光能的充分利用,通过积累可溶性糖提高渗透势,从而增强植物的耐旱能力。综合分析各项生理生态参数,蓝靛果忍冬不同品种苗均能耐受一定程度的干旱胁迫,各品种耐旱性强弱排序为伊利亚达>瓦休甘斯卡亚>北日勒>火红蛋白石>巴克恰尔斯卡亚>蔚蓝。本研究对选育适宜干旱、半干旱地区栽培推广的蓝靛果忍冬品种具有理论意义和实际价值。

The leaves of six kinds of Lonicera caeruleae Rehd varieties were taken as the experimental materials, and photosynthesis and physiological changes under the drought stress were measured in order to clarify the tolerance of Lonicera caeruleae Rehd varieties to drought stress. In this experiment, artificial potted natural drought method was applied to simulate normal drought stress, then photosynthesis and physiological changes under the four drought stress levels（ 10%, 20%, 30% and 40% ） were measured, as well as systemic comparison of adaptive changes of the parameters and its difference analysis . The results showed that with the intensification of drought stress, as the absolute soil moisture is 10% , the Pn of Yilyada was highest, 0.68μmol · m^-2 · s^ -1, while the Pn of Berel and Lazurnaya was negative, and others were also decline, and there was a significant difference with the absolute water content of soil at 40% （p≤0.01）. The drop of Tr and WUE were slow and decreased by 67.95% and 79.35% , respectively, compared with the absolute water content of soil at 40%; the Changes of Gi showed that the Pn decline of Yilyada and Bakqarckaya were caused by non - stomatal factor; With the extent of drought stress aggravating, chlorophyll content of Yilyada and Berelwere significantly higher than other varieties and increased by 37.4% , and 0. 62% in absolute water content of soil at 40%. Soluble sugar content were increased slowly, which showed that the two species could ensure full utilization of light energy by increasing the chlorophyll content under drought conditions, and accumulation of soluble sugars increased osmotic potential of the species, and enhanced the ability of the plant＇s drought tolerance. Comprehensive analysis of the physiological and ecological parameters, Lonicera caeruleae Rehd seedlings of different varieties can be drought- stress tolerant to certain degree, 6 kinds of Lonicera caeruleae Rehd varieties drought resistance forces followed the order of Yilyada 〉 Vacugancksya 〉 Berel 〉 Ogneny opal 〉 Bakqarskaya 〉 Lazurnaya. Research on drought resistance of Lonicera caeruleae Rehd provided theoretical and practical value for breeding and cultivating in drought, and semi-arid regions.