正交试验法优选蚕茧中丝胶蛋白的提取工艺

Optimization of Extraction Technology for Sericin from Silkworm Cocoon with Orthogonal Design

目的:优选蚕茧中丝胶蛋白的最佳提取工艺。方法:以丝胶蛋白提取率和丝胶蛋白含量为考察指标,采用单因素试验和正交试验设计,优选蚕茧中丝胶蛋白的最佳提取工艺。结果:蚕茧中丝胶蛋白的最佳提取工艺为:30倍量1%Na2CO3溶液提取2次,每次3 h,提取温度为100℃。蚕茧中丝胶蛋白的提取率可达27.1%。结论:优化得到的提取工艺稳定、可行,可为进一步研究蚕茧中丝胶蛋白的药理药效提供参考。

Objective：To optimize the appropriate extracting technology for sericin from Silkworm cocoon. Methods： Using sericm extraction rates and sericin content as the indices. The single and orthogonal experiments were used to determine the best conditions. Results ： The optimal extraction technology for sericin from Silkworm cocoon was as follows ： 1 ： 30 for the ratio of solid to liq- uid,3 h reflux for 2 times of extraction and water temperature at 100 ~C. The extraction rate of sericin from Silkworm cocoon was 27. 1%. Conclusion：The optimal extraction technology is stable, feasible, and can provide reference for further pharmacological study on cocoon seriein.