摘要
目的:优选蚕茧中丝胶蛋白的最佳提取工艺。方法:以丝胶蛋白提取率和丝胶蛋白含量为考察指标,采用单因素试验和正交试验设计,优选蚕茧中丝胶蛋白的最佳提取工艺。结果:蚕茧中丝胶蛋白的最佳提取工艺为:30倍量1%Na2CO3溶液提取2次,每次3 h,提取温度为100℃。蚕茧中丝胶蛋白的提取率可达27.1%。结论:优化得到的提取工艺稳定、可行,可为进一步研究蚕茧中丝胶蛋白的药理药效提供参考。
Objective:To optimize the appropriate extracting technology for sericin from Silkworm cocoon. Methods: Using sericm extraction rates and sericin content as the indices. The single and orthogonal experiments were used to determine the best conditions. Results : The optimal extraction technology for sericin from Silkworm cocoon was as follows : 1 : 30 for the ratio of solid to liq- uid,3 h reflux for 2 times of extraction and water temperature at 100 ~C. The extraction rate of sericin from Silkworm cocoon was 27. 1%. Conclusion:The optimal extraction technology is stable, feasible, and can provide reference for further pharmacological study on cocoon seriein.
出处
《中药材》
CAS
CSCD
北大核心
2015年第5期915-918,共4页
Journal of Chinese Medicinal Materials
基金
河北省自然科学基金(H2013406096)
关键词
蚕茧
丝胶蛋白
正交试验
提取工艺
Silkworm cocoon
Sericin
Orthogonal design
Extraction technology