制黄草乌化学成分研究

Chemical Constituents from Processed Products of Aconitum Vilmoriniani Radix

目的：对制黄草乌的化学成分进行研究。方法：采用硅胶、氧化铝和反相C18柱色谱及重结晶等方法进行分离纯化,通过质谱、核磁共振技术和理化性质进行结构鉴定。结果：从制黄草乌的甲醇提取物中分离得到10个化合物,分别鉴定为：滇乌碱（1）、8-去乙酰基滇乌头碱（2）、geniculatine C（3）、黄草乌碱乙（4）、黄草乌碱丙（5）、黄草乌碱丁（6）、talatisamine（7）、β-谷甾醇（8）、β-胡萝卜苷（9）、β-谷甾醇乙酸酯（10）。结论：化合物1~10均为首次从制黄草乌中分离得到。

Objective： To investigate the chemical constituents of the processed products of Aconitum Vilmoriniani Radix. Methods：The constituents were isolated by repeated column chromatography over silica gel,alumina and RP-C18 as well as recrystallization. The structures were elucidated on the basis of spectral analysis and physicochemical properties. Results： Ten compounds were obtained from the methanol extract,and they were identified as yunaconitine（ 1）,8-deacetyl-yunaconitine（ 2）,geniculatine C（ 3）,vilmorrianine B（ 4）,vilmorrianine C（ 5）,vilmorrianine D（ 6）,talatisamine（ 7）,β-sitosterol（ 8）,β-daucosterol（ 9） and β-sitosterol acetate（ 10）. Conclusion： All compounds are obtained from the processed products of Aconitum Vilmoriniani Radix for the first time.