基于代谢组学的刺五加多糖对大鼠心脏内源性物质代谢的影响

Effect of Acanthopanax senticosus Polysaccharides on Cardiac Endogenous Metabolism in Rats Based on Metabolomics

目的:运用代谢组学方法考察刺五加多糖对正常生理状态大鼠内源性物质代谢的影响,寻找潜在的生物标志物并分析其代谢途径,探讨其药理作用及作用机制。方法:SD大鼠随机分为空白对照组和刺五加多糖给药组,每组10只,分别给予生理盐水和刺五加多糖,连续灌胃20 d,于第21天取心肌组织,经处理供UPLC-Q-TOF/MS分析。结果:鉴定出20个潜在生物标志物,6条主要代谢通路,与心脑血管等疾病有关。结论:刺五加多糖对心脑血管疾病、癌症等疾病有一定的药理作用,其作用机制可能涉及氨基酸代谢、脂肪酸代谢及叶酸代谢等代谢过程。

Objective ： Using metaholomies method to study the influence of Acanthopanax senticosus polysaeeharides on cardiac en- dogenous metabolism in rats,in order to find potential biomarkers and analyze the metabolic pathways which can explore the pharmaco- logical effects and mechanisms of action. Methods ：20 SD rats were randomly divided into two groups, the blank and Acanthopanax senti- cosus polysaeeharides treatment groups,which were treated with saline and Acanthopanax senticosus polysaecharide for 20 days. On the 21 th day,heart tissue were collected and each sample extract was analyzed by UPLC-Q-TOF/MS. Resuhs ：20 potential biomarkers and 6 major metabolic pathways related to cardiovascular and eerebrovaseular diseases were identified. Conelusion：Acanthopanax senticosus polysaeeharides has a certain pharmacological effects on eardio-eerebro vascular diseases,cancer and other diseases. Its mechanism may be related to the metabolic process of amino acids, fatty acid and folate.