石菖蒲挥发油联合人参总皂苷对D-半乳糖联合氯化铝致阿尔茨海默病模型小鼠学习记忆能力及脑组织细胞凋亡的影响

Effects of Total Ginsenosides and Volatile Oil of Acorus tatarinowii Co-Administration on Ability of Learning and Memory and Apoptosis in Alzheimer's Disease Mice Model Induced By D-Galactose and Aluminium Chloride

目的:研究石菖蒲挥发油联合人参总皂苷对D-半乳糖联合氯化铝致阿尔茨海默病(Alzheimer's disease,AD)模型小鼠的学习记忆能力及脑组织细胞凋亡的影响。方法:将50只昆明小鼠随机分为正常对照组、模型组、安理申组(1 mg/kg)、定志丸组(10 g/kg)和石菖蒲挥发油+人参总皂苷组(石菖蒲挥发油30 mg/kg,人参总皂苷150 mg/kg)。除正常对照组外,各组小鼠通过腹腔注射D-半乳糖(150 mg/kg)并灌胃氯化铝(5 mg/kg)制备AD模型,连续40 d,1次/d。在造模第20天开始各组小鼠同时给予相应药物治疗,连续给药40 d,1次/d。用水迷宫实验和避暗实验来考察小鼠学习记忆能力;ELISA检测皮质和海马Aβ1-42和BCL-2的含量,紫外分光光度法检测皮质和海马乙酰胆碱酯酶(ACh E)和乙酰胆碱转移酶(Ch AT)的活性,WST-1法检测皮质和海马超氧化物歧化酶(SOD)活性,TBA法检测皮质和海马丙二醛(MDA)含量;免疫组化法观察Aβ1-42沉淀形成的老年斑情况;HE染色观察脑组织形态。结果:与模型组比较,石菖蒲挥发油+人参总皂苷组AD小鼠的游泳时间、盲区数和错误次数显著减少(P<0.05),潜伏期时间显著延长(P<0.05);皮质和海马中的ACh E活性增强、Aβ1-42和MDA的含量显著降低(P<0.05或P<0.01),Ch AT和SOD活性、BCL-2含量显著升高(P<0.05或P<0.01);老年斑形成减少,脑组织形态明显改善。结论:石菖蒲挥发油联合人参总皂苷能提高AD小鼠学习记忆能力并抑制脑组织细胞凋亡。

Objective： To observe the effects of the co-administration of total ginsenosides and volatile oil of Acorus tatarinowii on the ability of learning and memory and apoptosis in Alzheimer＇s disease（ AD） mice model induced by D-galactose and aluminium chloride. Methods： 50 Kunming（ KM） mice were randomly divided into normal group,model group,Aricept group（ 1 mg / kg）,Ding Zhi Wan group（ 10 g / kg） and co-administration of total ginsenosides and volatile oil of Acorus tatarinowii group（ co-administered group,the doses of volatile oil of Acorus tatarinowii and total ginsenosides were 30 mg / kg and 150 mg / kg,respectively）. In addition to normal group,mice in other groups were given D-galactose 150 mg /（ kg·d）,ip,and aluminium chloride 5 mg / kg,ig,once daily for 40 days. At the same time,mice in the treated groups were administrated with the corresponding drug from the 20 th day after the modeling,once daily for 40 days. Water maze and avoiding darkness experiments were used to test learning and memory abilities; Aβ1-42 and BCL-2 content in cortex and hippocampus were detected by ELISA; the vitalities of acetyl cholinesterase（ ACh E） and acetylcholine transferase（ Ch AT）were detected by ultraviolet spectrophotometry. Superoxide dismutase（ SOD） vitalities were detected by a water-soluble tetrazolium salt（ WST-1） method; the content of malondialdehyde（ MDA） in cortex and hippocampus were detected by the thiobarbituric acid（ TBA）method; senile plaque on Aβ1-42 precipitation were observed by immunohistochemistry; brain tissues were observed by hematoxylin-eosin staining（ HE）. Results： As compared with model group,in the co-administered group,the time of AD mice swimming,the numbers of blind area and electric shock reduced significantly（ P 0. 05）,and the latent period was prolonged（ P 0. 05）; ACh E activity and levels of Aβ1-42 and MDA in cortex and hippocampus were decreased significantly（ P 0. 05 or P 0. 01）; Ch AT and SOD activities as well as BCL-2 content were increased significantly（ P 0. 05 or P 0. 01）; the formation of senile plaque was decreased and brain tissue morphology was improved. Conclusion： Total ginsenosides and volatile oil of Acorus tatarinowii co-administration has an effect on improving the ability of learning and memory and inhibiting apoptosis.