气质联用-模式识别对甲嘎松汤质量控制的方法研究

Pattern Recognition Analysis for Quality Control of Jia Ga Song Tang by GC-MS

目的:建立藏方甲嘎松汤的质量评价方法。方法:通过水蒸气蒸馏法提取甲嘎松汤挥发油样品,采用气相色谱-质谱(GC-MS)联用技术对挥发油进行分析,并转化成数据矩阵,采用主成分分析以及系统聚类分析进行化学模式识别研究。结果:经聚类分析可将16批甲嘎松汤挥发油样品分为2大类,经主成分分析可将甲嘎松汤综合为3个主成分,可分为2大类;2种模式识别方法对不同甲嘎松汤挥发油样品能准确识别,且其识别结果一致。结论:气质联用-模式识别是一种科学、准确、有效的甲嘎松汤质量控制和评价方法。

Objective：To establish a scientific method for identification and evaluation of the Tibetan prescription Jia Ga Song Tang. Methods ： Volatile oil was extracted by water steam distillation and analyzed by GC-MS. Principal component analysis （ PCA ） and hierarchical cluster analysis （HCA）were applied to the samples for chemical fingerprint pattern recognition research. Resuhs ： 16 samples according to hierarchical cluster analysis （HCA）and principal component analysis （PCA）were divided into two classes, and results from two recognition analysis methods had good consistency. Conclusion ： GC-MS-pattern recognition method was a kind of scientific, accurate and effective method for the quality evaluation of Jia Ga Song Tang.