醋延胡索饮片、水煎液、配方颗粒高效液相色谱特征图谱相关性研究

Correlation of HPLC Characteristic Spectra of Vinegar Corydalis Rhizoma Decoction Pieces,Water Decoction and Formula Granules

目的:采用HPLC法对醋延胡索饮片、水煎剂和配方颗粒的特征图谱进行相关性研究,考察三者之间主要化学成分的转移情况。方法:采用菲罗门Gemini C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-1%醋酸-醋酸铵缓冲溶液(p H=6.0),梯度洗脱,流速为0.8 m L/min,柱温为30℃,检测波长为280 nm。分别建立了醋延胡索饮片、水煎液、配方颗粒的特征图谱。结果:醋延胡索配方颗粒HPLC特征图谱的10个特征峰全部可在水煎剂中得到追踪,有9个可在饮片中得到追踪,并指认出原阿片碱、盐酸巴马汀、盐酸小檗碱、延胡索乙素4个成分。结论:醋延胡索饮片、水煎液和配方颗粒的主要化学成分组成基本相同,其共有成分含量比例相近。

Objective： To study the correlation of characteristic spectra of Vinegar Corydalis Rhizoma decoction pieces,water decoction and formula granules by HPLC,and to investigate the transfer of the main chemical constituents between three different forms. Methods： The analysis was carried out by a Phenomenex Gemini C18column（ 250 mm × 4. 6 mm,5 μm） with acetonitrile-1% acetic acid and ammonium acetate buffer solution（ p H 6. 0） as the mobile phase in a gradient elution mode. The detection wavelength was280 nm with a flow rate of 0. 8 m L / min. The column temperature was 30 ℃. The characteristic spectra from 11 batches of Vinegar Corydalis Rhizoma decoction pieces,11 batches of water decoction and 11 batches of formula granules were established respectively. Results： Ten peaks in the HPLC characteristic spectra from 11 batches of formula granules could be tracked in the water decoction,nine peaks in the HPLC characteristic spectra could be tracked in the decoction pieces. In the ten common peaks,four components such as protopine,palnatine chloride,berberine hydrochloride and tetrahydropalmatine were verified. Conclusion： The main chemical components of Vinegar Corydalis Rhizoma decoction pieces,water decoction and formula granules are basically the same,the common component contents have similar proportion.