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双相层流微流控芯片快速提取人参皂苷 被引量:4

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摘要 目的:建立基于微流控芯片液-液萃取技术进行人参药材样品前处理的方法。方法:注射泵引入药材初提液及萃取剂于芯片中进行萃取,收集样品溶液进行HPLC检测。对芯片的前处理;药液与萃取剂的流速、流速比;萃取通道的宽度等因素进行考察。结果:纯水清洗后风干的芯片,药液与萃取剂的流速与流速比为v(水饱和正丁醇)∶v(药材初提液)=6(μL/min)∶12(μL/min),萃取通道宽度为200μm的情况下萃取效果更理想。在优化条件下平行试验6次,RSD小于1.2%。芯片萃取所得人参皂苷Rg1、Re、Rb1含量分别为1.05、0.54、1.30 mg/m L,萃取时间仅需2 min,与传统液液萃取相比,芯片萃取的萃取率分别提高了12%、13%、15%。结论:该方法简单、快速、萃取效率高。
出处 《中药材》 CAS CSCD 北大核心 2015年第5期1070-1072,共3页 Journal of Chinese Medicinal Materials
基金 国家自然科学基金(81001600)
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参考文献8

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