miR-155在急性胰腺炎患者外周血中的表达及其临床意义

Clinical significance of serum mi R-155 expression level in acute pancreatitis patients

目的:探讨急性胰腺炎(acute pancreatitis,AP)患者血清中micro RNA155(mi R-155)的表达水平及其临床意义.方法:以28例中/重度急性胰腺炎患者为A组、32例轻度急性胰腺炎患者为B组、20例健康者为对照组,取血标本进行血清RNA抽提,采用实时荧光定量PCR(RT-q PCR)技术检测血清中mi R-155表达量,分析mi R-155的表达及与各组AP患者临床特征的关系,并绘制ROC曲线分析其诊断AP的价值.结果:miR-155相对表达量:A组为8.09±2.28,B组为4.94±1.41,对照组为1.58±0.81,差异均有统计学意义(P<0.05);mi R-155与MCTSI评分、BISAP评分、Ranson评分,APACHEII评分有相关性(P<0.05),与性别、年龄无相关性(P>0.05);mi R-155的ROC曲线下面积0.882,预测AP的最佳mi R-155浓度截断值为3.55,灵敏度92.5%,特异度85.7%.结论:mi R-155可能是AP患者的血清标志物,且对AP严重程度有一定的预测作用.

AIM：To determine the serum expression level of miR-155 in acute pancreatitis（AP） patients and to analyze its clinical significance.METHODS：Twenty-eight patients with moderately severe AP（MSAP） or severe AP（SAP） were included in group A,32 patients with mild AP（MAP） were included in group B,and 20 healthy people were included as normal controls（NCs）.Serum miR-155 expression levels were detected by real-time quantitative PCR.The differences in miR-155 expression level in different groups,and the correlation between miR-155 expression level and clinical characteristics of AP patients were analyzed.A receiver operating characteristic curve（ROC curve） was plotted to explore the diagnostic value of serum miR-155.RESULTS：The mean relative expression level of serum miR-155 was 8.09 ± 2.28 in group A,4.94 ± 1.41 in group B,and 1.58 ± 0.81 in the NC group,and there were significant differences between groups A and B,group A and the NC group,and group B and the NC group（P〈 0.05）.The relative expression levels of miR-155 were correlated with patients＇ BISAP score,Ranson score,APACHE II score and MCTSI score（P〈 0.05）,but not with patients＇ gender and age.The area under the ROC curve of miR-155 for prediction of AP severity was 0.882,with a sensitivity of 92.5%and specificity of 85.7%,and the best cutoff value was 3.55.CONCLUSION：Serum miR-155 may be a potential biomarker for the diagnosis of AP,and it is clinically useful for the prediction of the severity of AP.