黄花棘豆2种生物碱成分的鉴定

Alkaloids from Poisonous Plant Oxytropisochrocephala

为分析我国天然草地主要有毒植物黄花棘豆的生物碱成分,为其毒理学、药理学和资源综合利用提供理论依据,本试验采用硅胶柱层析、Sephadex LH-20、RP18、半制备HPLC技术和现代波谱技术对黄花棘豆生物碱成分进行研究。结果表明,从黄花棘豆正丁醇萃取部位分离得到2个极性较大的生物碱成分,通过现代波谱技术鉴定为(+)-9α-羟基苦参碱和(-)-9α-羟基槐果碱。2种化合物均为首次从棘豆属植物中分离得到。

In order to make clear the alkaloid constituents of poisonous plant Oxytropis ochrocephala dis-tributed in natural grassland and provide the further theoretical basement for toxicology,pharmacology and comprehensive utilization of this plant,the BuOH exact of Oxytropis ochrocephala was purified by means of various chromatographic techniques such as silica gel column,Sephadex LH-20 gel,RP-18 and semipreparative HPLC.As the result,two alkaloids were obtained and their structures were elucidated by spectral analysis as (+)-9α-hydroxymatrine (1),(-)-9α-hydroxysophocarpine (2).Compounds 1-2 were i-solated from the plants genus Oxytropis for the first time.