藻源型溶解性有机氮的产生及不同时期藻类有机物的特性

Formation of algae-derived DON and characterization of algae organic matter(AOM) from different stages

研究了铜绿微囊藻藻细胞全生命周期中氮的转化过程以及藻类有机物的性质,结果表明:溶解性有机氮(DON)在藻类生长周期中总体呈现上升趋势,藻细胞在其生长初期释放量小于0.05mg DON/107cells,衰亡期释放能力约为0.40mg DON/107cells;胞外有机物(EOM)中分子量分布在对数期和衰亡期以<1k Da和>100k Da为主,稳定期以<30k Da为主;胞内有机物(IOM)的分子量分布随藻的生长变化较小,集中在<1k Da和>100k Da范围内.三维荧光光谱分析表明,EOM中荧光物质主要为类腐植酸和溶解性微生物代谢产物;而IOM主要由类芳香蛋白和溶解性微生物代谢产物组成.

Formation rules of dissolved organic nitrogen （DON）, as well as characteristics of extracellular oganic matter （EOM） ＆ intracellular organic matter （IOM） during the growth of Microcystis aeruginous have been studied. The results show as follows： DON concentration of EOM shows a growing tendency during the growth ofMicrocystis aeruginous, the yield of which is less than 0.05mg DON per 10^7cells in the early growth phase, around 0.40mg DON per 10^7cells in the late growth phase, respectively; molecular weight distributions of EOM during exponential phase and decline phase mainly concentrate in range of less than lkDa and more than 100kDa, while organic matter less than 30kDa dominating EOM in stationary phase;molecular weight distributions of IOM concentrate in range of more than lkDa and less than 100kDa in the three phases. The main fluorescence regions of EOM occur at the fluorescence region of humic acid-like materials and soluble microbial products. By contrast, the region of aromatic proteins and soluble microbial products dominate the main region of EEM spectra of IOM.