纳米铜粉对中肋骨条藻的毒性效应

Inhibition effects of copper nanoparticles on the growth of Skeletonema costatum

以中肋骨条藻作为受试藻种,研究铜离子、纳米铜和微米铜对它的毒性效应.结果表明,3种铜试剂在不同程度上都会抑制中肋骨条藻的生长,藻密度和叶绿素的增加值与铜试剂加入量基本呈负相关.在整个实验周期96h内,当Cu2+浓度大于0.1mg/L时会对藻产生很大的抑制作用,而纳米铜浓度大于0.15mg/L会严重影响藻密度和叶绿素,在24h内当微米铜浓度低于2mg/L时会促进藻的生长,在96h时高浓度(>1mg/L)会抑制其生长.纳米铜对中肋骨条藻的毒性作用一方面是溶出的铜离子,另一方面是纳米粒子本身.

By using Skeletonema costatum as experimental algae, the toxic effect of Cu^2＋, nanometer-copper, and micrometer-copper on it was studied. The results showed that the three copper reagents could inhibited the growth orS. costatum in different degrees. Generally, the increase value of chlorophyll and algal cell density had negative relationship with addition of the copper reagents. In the whole experimental period （96hours）, the growth of S. costatum was strongly inhibited if the concentration of Cu^2＋ was higher than 0.1mg/L, and the algal density and chlorophyll were seriously affected as the concentration of nano-copper was higher than 0.15mg/L. Micro-copper stimulated the growth of S. costatum within 24h when its concentration was lower than 2mg/L. Algae growth was inhibited within 96h in high concentration of micro-copper （〉1mg/L）. The mechanism of the toxic effect of copper nanoparticles on Skeletonema costatum was attributed to its dissolved copper ions and the nanoparticles itself.