microRNA-148b在肝细胞癌组织中的表达及其临床意义

Expression of MicroRNA-148b in Hepatocellular Carcinoma Tissue and Its Clinical Significance

目的:探讨肝细胞癌(hepatocellular carcinoma,HCC)组织中microRNA-148b(miR-148b)的表达与HCC预后的关系。方法:从上海中医药大学附属曙光医院冰冻组织样本库中调取2007—2010年手术切除的HCC组织及相应癌旁组织标本,采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)法检测组织标本中miR-148b的表达情况,结合临床病理指标及生存资料,分析miR-148b表达改变的临床意义。结果:与癌旁组织比较,HCC组织中miR-148b表达水平显著降低(P<0.01)。miR-148b低表达与HCC血管侵犯及TNM分期等恶性临床病理指标相关。Kaplan-Meier生存曲线分析显示,miR-148b低表达组患者总生存率较低。结论:人HCC组织中miR-148b表达下降,且与HCC不良的临床预后有关。

Objective：To investigate the relationship between the expression of microRNA-148b（miR-148b） in human hepato cellular carcinoma （HCC） and the prognosis of HCC. Methods： The specimens of HCC tissue and para-carcinoma tissue ac- quired by surgical resection during 2007 and 2010 were collected from frozen tissue specimen library of Shuguang Hospital Af- filiated to Shanghai University of Traditional Chinese Medicine. The expression of miR-148b in tissue specimen was detected by real-time quantitative polymerase chain reaction （RT-qPCR）. The clinical significance of expression change of miR-148b was analyzed by considering the clinical pathological indicators and survival data. Results： The expression level of miR-248b in HCC was significantly lower than that in para-tumor tissues（P〈0. 01）. Low expression of miR-148b was associated with HCC ma- lignant clinical pathological indicators such as vascular invasion and TNM stage. Kaplan-Meier survival curve analysis showed that the patients with low expression of miR-148b had poor overall survival rate. Conclusions.. The expression of miR-148b de- creases in human HCC tissue. And it is associated with the poor clinical prognosis.