白花玫瑰植物组织培养初探

Preliminary study on tissue culture of White Rose

以白花玫瑰幼嫩茎段为外植体,对其离体培养途径进行研究,建立白花玫瑰的组织培养体系。结果表明:芽诱导培养基配方为:MS+6-BA 2.5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L+琼脂6 g/L;增殖培养基配方为:MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂6 g/L;生根培养基配方为1/2MS+NAA 0.2 ml/L+蔗糖30 g/L+琼脂6 g/L。

The tissue culture system of White Rose was studied by using tender stem segments as explant.The results show that the bud inducing culture medium formula was MS＋2.5 mg/L 6-BA＋0.3 mg/L NAA＋30 g/L sucrose＋6 g/L agar; The proliferation culture formula was MS＋3.0 mg/L 6-BA＋0.2 mg/L NAA＋30 g/L sucrose＋6 g/L agar; The rooting cultural medium formula was 1/2 MS＋0.2 mg/L NAA＋30 g/L sucrose＋6 g/L agar.