南瓜蛋白联合常用化疗药物对人胰腺癌BxPC-3细胞的体外抑制作用

Cucurmosin combined with common chemotherapeutic drugs inhibited human pancreatic cancer cell line BxPC-3 in vitro

目的研究南瓜蛋白（CUS）联合胰腺癌常用化疗药物吉西他滨（GEM）、氟尿嘧啶（5-FU）、紫杉醇（erx）、顺铂（DDP）对人胰腺癌细胞株BxPC-3的增殖抑制作用。方法采用磺酰罗丹明B（SRB）比色法测定CUS分别与GEM、5-FU、PTX、DDP联用对体外培养的BxPC-3细胞的增殖抑制作用；应用集落形成实验测定CUS分别与GEM、5-FU、PTX、DDP联用对体外培养的BxPC-3细胞集落形成的影响；用金氏公式判断药物联用的协同效果。结果CUS联用GEM、5-FU、HIX、DDP对BxPC．3细胞的增殖抑制率均大于各药单用的抑制率，q〉0．85；CUS与胰腺癌常用化疗药物均在低浓度范围内联用的相加效果明显。CUS联用GEM、5-FU、PTX、DDP对BxPC-3细胞的集落形成抑制率均大于各药单用的抑制率，q〉1．15。结论CUS联合GEM、5-FU、PTX、DDP常用化疗药物对体外培养的BxPC-3细胞的生长抑制具有较好的协同作用。

Objective To investigate the inhibitory effect of cucurmosin （CUS） combined with the commonly used chemotherapeutic drugs for pancreatic cancer in clinical practice including Gemcitabine （GEM） , Fluorouracil （5-FU） , Paclitaxel （PTX） and Cisplatin （DDP） on cell proliferation of human pan- creatic cancer cell line BxPC-3. nehtods Sulforhodamine B （SRB） assay was used to detect the inhibition on the cell proliferation of BxPC-3 cells in vitro after the treatment of CUS combined with GEM, 5-FU, PTX and DDP, respectively. Colony formation assay was also conducted and Jin＇ s formula was used to assess the synergistic effect of these combinations. Results The inhibition rate of CUS combined with GEM, 5-FU, PTX and DDP were all higher than those of each drug alone （ q 〉 0.85 ） , which became obvious in low con- centrations. The colony formation inhibition rate of CUS combined with GEM, 5-FU, PTX or DDP were all higher than each single drug treatment （q 〉 1.15）. Conclusion CUS could enhance the cell growth inhibi- tion of GEM, 5-FU. PTX and DDP in BxPC-3 cells in vitro with a good svnergistic effect.