海带有机碘与无机碘对甲状腺功能亢进Wistar大鼠心肌ATP酶的影响

Contrastive study on the effect of 3, 5-diiodotyrosine and potassium iodide on myocardial ATPase in hyperthyroidism Wistar rats

目的观察海带有机碘（DIT）与无机碘（KI）对甲状腺功能亢进（简称甲亢）形成中的wistar大鼠心肌ATP酶活性的影响。方法雄性Wistar大鼠72只，按体质量采用随机数字表法分成8组（每组9只），分别为对照组，甲亢模型组，DIT和KI低、中、高剂量组（DIT和KI均为25．0、166．7、500．1μg／kg）。对照组大鼠灌胃生理盐水；甲亢模型组灌胃甲状腺片悬浊液（200．0mg／kg）；DIT和KI低、中、高剂量组在给予相应剂量的碘同时灌胃甲状腺片悬浊液。每天灌胃1次，连续1个月，断头处死各组大鼠取心脏组织，比色法检测各组大鼠心肌钠-钾（Na＋-K＋）-ATP酶、镁离子（M矿）．ATP酶、钙离子（Ca2＋）-ATP酶的活性。结果各组间大鼠心肌Na＋-K＋-ATP酶、Mg2＋．ATP酶、Ca扯ATP酶活性比较差异均有统计学意义（F=2．99、3．03、6．18，P均〈0．01）。其中与对照组[（4．01±0．22）、（4．28±0．28）、（4．46±0．35）μmol／mg·h]比较，甲亢模型组3种心肌ATP酶活性[（3．60±0．25）、（3．42±0．31）、（3．85±0．17）μmol／mg·h]显著降低（P均〈0．01）；DIT中剂量组Mg2＋-ATP酶[（3．89±0.35）μmol／mg·h]和中、高剂量组Ca2＋-ATP酶[（4．12±0．20）、（4．09±0．21）μmol／mg·h]活性降低（P均〈0．05）；KI低、中、高剂量组Na＋-K＋-ATP酶[（3．64±0．32）、（3．60±0．32）、（3．53±0．33）μmol／mg·h]、Ca2＋-ATP酶[（3．93±0．22）、（3．90±0．23）、（3．85±0．26）μmol／mg·h]，高剂量组Mg^2＋-ATP酶活性[（3．65±0．49）μmol／mg·h]显著降低（P〈0．05或〈0．01）。与甲亢模型组比较，DIT低、中剂量组Mg2＋-ATP酶[（4．06±0．51）、（3．89±0．35）μmol／mg·h]活性，低、中、高剂量组Ca2＋-ATP酶[（4．15±0．26）、（4．12±0．20）、（4．09±0．21）μmol／mg·h]活性升高（P均〈0．05）。结论大鼠在甲亢形成过程中补充相同剂量的碘剂时，DIT对大鼠心肌损害要小于同剂量的KI。

Objective To study the effects of 3, 5-diiodotyrosine （DIT） and potassium iodide （KI） on myocardial ATPase activity in hyperthyroidism Wistar rats induced by thyroid tablets. Methods Seventy-two Wistar rats were divided into 8 groups according to body weight by the random number table method （9 rats in each group）, respectively, which were control group, hyperthyroidism model group, low, medium and high doses groups （both DIT and KI contents were 25.0, 166.7, 500.1μg/kg）. Physiological saline was intragastrically administrated to the control group; the hyperthyroidism model group was given thyroid tablet suspension （200.0 mg/kg）; DIT and KI groups were given thyroid tablet suspension with corresponding doses of iodine simultaneously. The medicine was given once a day for a mouth, all the rats were sacrificed and heart tissue was collected. The colorimetric method was used to examine the activity of ATPases （Na＋-K2-ATPase, Mg2＋-ATPase, Ca2＋-ATPase）. Results The activities of Na＋-K＋-ATPase, Mg2＋-ATPase, Ca2^-ATPase were significantly different statistically between groups （F = 2.99, 3.03, 6.18, all P 〈 0.01）. Compared with the control group [（4.01±0.22）, （4.28±0.28）, （4.46±0.35） μmol/ mg·h], the activities of ATPases （Na2-K＋-ATPase, Mg2＋-ATPase, Ca2＋-ATPase included） were reduced significantly in hyperthyroidism model group [（3.60 ±0.25）, （3.42±0.31）, （3.85 ±0.17）μmol/mg.h, all P 〈 0.01];the activities of Mg2＋-ATPase in DIT medium dose group [（3.89±0.35）μmol/mg-h], Ca2＋-ATPase in DIT medium and high doses groups [（4,12±0.20）, （4.09±0.21）μmol/mg-h] were reduced significantly （all P 〈 0.05）; the activities of Na＋-K＋-ATPases, Ca2＋- ATPase were decreased significantly in three KI groups [（3.64±0.32）, （3.60±0.32）, （3.53± 0.33）, （3.93±0.22）, （3.90±0.23）, （3.85±0.26）μmol/mg.h], Mg2＋-ATPase in KI high dose group [（3.65±0.49）μmol/mg.h] was decreased significantly （P 〈 0.05 or 〈 0.01）. Compared with the hyperthyroidism model group, the activities of ATPase were increased in most of the DIT groups [Mg2＋-ATPase in low, medium doses groups： （4.06μ0.51）, （3.89μ0.35）μmol/mg.h; Ca2＋-ATPase in low, medium, high doses groups （4.15±0.26）, （4.12±0.20）, （4.09±0.21）μmol/mg-h, all P 〈 0.05]. Conclusion Supplementation of thyroid tablets in the process of hyperthyroidism formation in Wistar rats will reduce myocardial damage by DTr compared with the same dose of KI.