扩张型心肌病患者血清差异蛋白表达及蛋白生物标志物研究

Differential protein expression and protein biomarker of dilated cardiomyopathy

目的观察扩张型心肌病（DCM）患者和健康人血清差异蛋白表达，筛选蛋白标志物，为DCM的诊断提供依据。方法2011年3—5月，在山东大学齐鲁医院、济南市中心医院和济南市第一人民医院心内科选择29例DCM住院患者作为DCM组，并以济南市当地健康人30例作为对照组。采集观察对象血清．采用液体芯片飞行时间质谱技术检测血清差异蛋白表达，使用ClinProTools2．2获取蛋白峰数据，建立ClinPrott判别模型，并筛选蛋白生物标志物。结果共获得73个蛋白质／多肽有效峰图，其中有57个差异蛋白／多肽．与对照组比较，DCM组有35个差异蛋白／多肽上调，22个差异蛋白／多肽下调。筛选出质荷比（m／z）值为4247．95、4209．37、1058．69、1074．78、2364．71的5个峰为蛋白生物标志物，经验证ClinPrott判别模型的敏感性与特异性分别为99．14％、99．16％。结论DCM患者血清蛋白差异表达，筛选出5个标志蛋白可作为DCM诊断与鉴别诊断的生物标志物。

Objective To provide more valuable information for diagnosis of dilated cardiomyopathy （DCM）, we detected differentially expressed proteins in serum from patients with DCM and healthy people and protein biomarkers were selected. Methods During the period from march 2011 to may, a total of 29 samples of resident patients with DCM from Qilu Hospital of Shandong University, Jinan Central Hospital and Jinan First Peoples Hospital and 30 local healthy people in Jinan were selected as DCM and control groups, respectively. Serum samples from these patients with DCM and controls were detected by ClinProt MALDII-TOF-MS. ClinProTools 2.2 software was used to get mass spectrometric data. The ClinPrott discrimination model was established to screen out differentially expressed proteins as potential biomarkers. Results Via comparing proteins/polypeptides peaks of DCM patients and healthy controls, 57 of all 73 peaks were found to be significantly different between the two groups. Compared with the control group, 35 peaks were up-expressed while the other 22 peaks were down- expressed. Five peaks were screened out as protein biomarkers. They were mass-to-charge ratio （m/z）： 4 247.95, 4 209.37, 1 058.69, 1 074.78, and 2 364.71. The sensitivity and specificity of ClinPrott discrimination model was 99.14% and 99.16%, respectively. Conclusion Patients with DCM have expressed serum proteins differently and we have found five protein markers which might have some value for diagnosis of DCM.