基于代谢调控深黄被孢霉产γ-亚麻酸培养基优化

Optimization of medium for the production of γ-linolenic acid by Mortierella isabellina based on metabolic regulation

研究深黄被孢霉产γ-亚麻酸过程的代谢调控,从中得出对深黄被孢霉产γ-亚麻酸有促进作用的营养成分,并利用Plackett-Burman实验对培养基营养成分进行筛选。得出葡萄糖、KNO3、乙酸钠、柠檬酸钠为影响产油量的关键因素。在此基础上,以γ-亚麻酸产量为响应值,采用响应面法优化,确定4个关键因素的最佳水平。综合Plackett-Burman实验和响应面法确定的最佳培养基配方为:葡萄糖100.90 g/L、KNO31.57 g/L、乙酸钠3.42 g/L、柠檬酸钠4.56 g/L、酵母膏3.75g/L、KH2PO42.25 g/L、Mg SO4·7H2O 0.60 g/L、Ca Cl20.30 g/L、Fe SO40.15 g/L、Zn SO40.20 g/L。在最佳条件下γ-亚麻酸的产量达1 525.20 mg/L,比初始产量725.90 mg/L提高了1.1倍。

The nutrients promoting the production of γ - linolenic acid by Mortierella isabeUina were found by studying on metabolic regulation of production process of γ - linolenic acid. The medium nutrients were screened by Plackett - Burman experiment. The results showed that the key factors influencing oil yield were glucose, KNO3, sodium acetate and sodium citrate. The four key factors were optimized by response surface methodology with yield of γ- linolenic acid as response value. Combining Plackett - Burman experiment with response surface methodology, the optimal medium formula were obtained as follows ： glucose 100.90 g/L, KNO3 1.57 g/L, sodium acetate 3.42 g/L, sodium citrate 4.56 g/L,yeast extract 3.75 g/L, KH2PO4 2.25 g/L, MgSO4· 7H20 0.60 g/L, CaCl2 0.30 g/L, FeSO40.15 g/L, ZnSO4 0.20 g/L. Under the optimal conditions, the yield of γ- linolenic acid reached 1 525.20 mg/L, increasing by 1.1 times than the initial yield （725.90 mg/L） .