响应面法优化混合培养微藻Chlorella sp.U4341油脂产率的研究

Research of the optimization of mixotrophic cultivation of lipid productivity by Chlorella sp.U4341 with response surface methodology

为了提高混合培养微藻Chlorella sp.U4341的油脂产率,采用响应面法对培养条件进行优化研究。通过单因素实验筛选出促进Chlorella sp.U4341油脂产率的最佳碳源、氮源分别为葡萄糖、Na NO3。利用Plackett-Burman实验筛选出显著影响Chlorella sp.U4341油脂产率的两个因子,通过最陡爬坡实验逼近最大油脂产率区域,运用中心组合实验及响应面分析确定了两个因子的最佳浓度:葡萄糖25.34 g·L-1,Na NO31.67 g·L-1。优化后的条件培养微藻Chlorella sp.U4341的油脂产率为280.57 mg·L-1·d-1,比优化前培养条件下提高了54%。

In order to increase the lipid productivity of microalgae Chlorella sp.U4341, optimization of mixotrophic cultivation condition was carried out by response surface methodology.The optimal carbon and nitrogen sources for Chlorella sp.U4341 were glucose and sodium nitrate, respectively.Two most important influencing factors for lipid productivity of Chlorella sp.U4341 were screened via Plackett-Burman experiment.Subsequently,the steepest ascent experiment was used to guide key factors to approach the maximum lipid productivity region. Optimal concentrations of the three influencing factors were confirmed by central composite design and response surface analysis,and the results were 2,5.34 g. L-1 of glucose, 1.67 g. L-1 of sodium nitrate.Cultivated with the optimized condition,the lipid productivity of Chlorella sp.U4341 reached 280.5？ mg. L-1 ·d-1 ,which was increased by 54% compared with the original cultivation condition.