响应面法优化纤维素酶协同提取沙棘籽粕原花青素的工艺研究

Optimization of Cellulase-assisted Extraction Process of Proanthocyanidins from Hippophae rhamnoides Seed Meal Using Response Surface Methodology

利用单因素试验及响应面法优化纤维素酶协同甲醇超声提取沙棘籽粕中原花青素的工艺。通过单因素试验筛选出提取时间、料液比、及提取液p H三个主要因素,以沙棘籽粕原花青素提取得率为响应因子进行Box-Behnken中心组合试验计划,建立沙棘籽粕原花青素提取得率的二次回归方程,得到最优提取条件。响应面法分析结果表明,最佳提取工艺参数为:超声功率250 W,室温下,酶用量为3%,提取时间55 min,料液比1∶30g/m L,p H值为5.0,提取3次。在此条件下,沙棘籽粕原花青素提取得率为3.07%。

In this study,ultrasonic-assisted extraction was developed to optimize the extraction process of pmanthocyani- dins （PC） presented in Hippophae rhamrwide~ seed meal. Ce]]ulase was introduced into the process for improving the ex- traction efficiency. The extraction conditions were optimized using response surface methodology （RSM） while extraction yield of PC were recorded as response and three key factors including extraction duration, ratio of solid to liquid and pH value of extraction solvent were selected by single-factor tests. Box-Behnken design and a quadric regression equation for predicting the extraction yield of PC were established and the optimized extraction conditions were determined as fol- lows：extraction duration as 55 rain,ratio of solid to liquid as 1：30 （g/mL） ,pH value of extraction solvent as 5.0 and times of extraction as 3 ,while ultrasonic power was set at 250 W,extraction temperature was set at 25 ~C with cellulose dosage of 3%. Under these optimized conditions,the extraction yield of PC was 3.07%.