摘要
以叉枝鸦葱为研究对象，用微波辅助提取法提取总黄酮，采用DPPH法研究叉枝鸦葱总黄酮对自由基的消除作用。同时，采用GraphpadPrism5．0软件进行动力学模型拟合。结果表明：叉枝鸦葱总黄酮对DPPH自由基具的清除作用大于芦丁和Vc，清除率为41．72％-87．72％，IC50％为5．6μ/mL。动力学模型拟合后所得方程Y=（YO—Ns）＊oxp（-K＊x）＋Ns，充分反映了叉枝鸦葱总黄酮对DPPH自由基的实际消除效应，在样品浓度为20μg／mL时效应关系最佳。
To investigate the antioxidant activity of total flavonoids in Scorzonera divaricata, microwave-assisted extraction method was used to extract total flavonoids. Anfioxidant activity was measured by DPPH free radial scavenging assay. Meanwhile, the dynamic model of response curves was simulated by Graphpacl Prism 5.0 software. The results showed that the total flavonoids had higher scavenging activity on DPPH free radial than Vc and rutin, clearance rote was 41. 72%-87.72%,ICs0 was 5.6μg/mL. A kinetics equation was eetablished：Y = （Y0-NS） ＊ exp （-K ＊ X） ＋ NS, which very well reflccted the efficiency of free radlcal scavenging. The appropriate concentration of the sample was 20 μg/mL.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第9期1616-1619,1600,共5页
Natural Product Research and Development
关键词
叉枝鸦葱
总黄酮
DPPH自由基
反应动力学
Scorzonera divaricata
total tlavonoids
DPPH free radical
kinetics of reaction
