间变少枝胶质细胞瘤血管内皮细胞1p／19q缺失与生存期的关系

Relationship between 1p/19q deletion of vascular endothelial cells in anaplastic oligodendroglioma and survival period

目的探讨间变少枝胶质细胞瘤（AO）血管内皮细胞1p／19q缺失情况，并分析其与生存期的关系。方法收集首都医科大学附属北京天坛医院2009年1月至2010年1月具有完整随访资料的原发性AO标本30例，其中男16例，女14例；年龄23～61岁，平均45．9岁。免疫荧光技术定位血管内皮细胞，荧光原位杂交法（FISH）检测1p／19q缺失状态。配对t检验统计血管内皮细胞1p／19q缺失率，生存分析法评估其与生存期的关系。结果AO血管内皮细胞的检测信号1p36缺失率显著高于其参照信号1q25（t=8．823，P〈0．001），检测信号19q13缺失率显著高于其参照信号19p13（t=8．316，P〈0．001）。生存分析结果显示：血管内皮细胞1p／19q共缺失的患者中位无进展生存期（PFS）及总体生存期（0s）均显著优于血管内皮细胞1p／19q未缺失的患者（PFS：X^2=16．199，P〈0．01；OS：X2=8．666，P〈0．01）。Cox回归分析结果表明血管内皮细胞1p／19q共缺失是独立预后因子（PFS：HR=0．063，95％CI：0．013—0．313，P=0．001；OS：HR=0．145，95％CI：0．310～0．678，P=0．014）。结论AO血管内皮细胞存在1p／19q缺失现象。血管内皮细胞1p／19q共缺失提示预后良好。

Objectives To investigate the lp/19q deletion status of vascular endothelial ceils in anaplastic oligodendroglioma （AO） and to analyze its relationship with survival period. Methods A total of 30 primary AO specimens with complete follow-up data from Beijing Tiantan Hospital, Capital Medical University were collected between January 2009 and January 2010. There were 14 females and 16 males, and their mean age was 45.9 years （ range 23 to 61 ）. Immunofluorescence technique was used to locate vascular endothelial cells. Fluorescence in situ hybridization （FISH） was used to detect the status of lp/19q deletion. The paired t test was used to count lp/19q deletion rate of vascular endothelial cells. Survival analysis method was used to assess its relationship with survival period. Results The detection signal l p36 deletion rate of AO vascular endothelial cells was significantly higher than its reference signal 1 q25 （t = 8. 823, P 〈 0. 001 ） ; the detection signal 19q13 deletion rate was signifcantly higher than its reference signal 19p13 （ t = 8. 316, P 〈 0. 001 ）. The results of survival analysis showed that both the median progression-free survival （PFS） and overall survival （OS） of the patients with lp/19q co-deleted of vascular endothelial cells were significantly better than those without lp/19q deletion of vascular endothelial cells （PFS ： X2 = 16. 199,P 〈 0.01 ; OS ： X2 = 8. 666, P 〈 0.01 ）. The result of Cox regression analysis showed that lp/19q co-deletion of vascular endothelial cells was an independent prognostic factor （PFS： HR = 0.063, 95% CI0.013-0.313, P=0.001; OS： HR=0.145, 95% C10.310-0.678, P=0.014）. Conclusions AO endothelial cells have a lp/19q deletion phenomenon, lp/19q co-deletion of vascular endothelial cells indicates that the prognosis will be good.