燕麦膳食纤维对食源性肥胖小鼠降脂减肥作用研究

Anti-Obesity Effect of Oat Dietary Fiber on High-Fat Diet-Induced Obese Mice

深入研究水溶性及水不溶性燕麦膳食纤维降脂减肥作用的效果。60只昆明小鼠,饲喂高脂饲料构建肥胖模型后,分别饲喂添加低、高剂量(7.33、14.67 g·kg-1(BW)·d-1)的燕麦水溶性(SDF)水不溶性(IDF)膳食纤维的高脂饲料作为试验组,另设饲喂普通饲料和高脂饲料作为正常对照组和肥胖模型组。测定相关指标的变化。与肥胖模型组相比,肥胖小鼠在食用燕麦膳食纤维以后,Lee’s指数显著下降(P<0.05),IDF效果最好;腹腔脂肪堆积显著减少(P<0.05),激素敏感性脂肪酶显著升高(P<0.05),同时胰岛素分泌减少促甲状腺激素分泌增加(P<0.05),SDF作用效果高于IDF(P<0.05);小鼠肝脏系数、肝脏总胆固醇(TC)和三酰甘油(TG)水平显著降低(P<0.05),肝脏肝酯酶(HL)和脂蛋白酯酶(LPL)活性升高(P<0.05),但是与正常组对照小鼠相比仍有显著差异(P<0.05),SDF效果比IDF更显著。燕麦SDF和IDF都具有降脂减肥作用,存在剂量效应,但作用途径不同。

This study evaluated the anti - obesity effect of soluble and insoluble oat dietary fiber on high - fat diet- induced obese mice. 60 mice were first fed with high -fat diet to establish obesity model. Then, obese mice were fed with high -fat diet that contains different doses（7.33 and 14.67 g· kg-1 BW d-1） of oat soluble （SDF） and insoluble （IDF） dietary fiber. Other mice fed with normal diet and high -fat diet were used as normal control （NC） group and obesity model group, respectively. Variation of related indexes was measured. It suggested that both SDF and IDF oat dietary fibers significantly decreased the Lee＇s Index （ P 〈 0.05 ）, especially for IDF. Besides, they could promote the fat breakdown （P 〈 0.05 ） and improve the activity of hormone sensitive lipase （ P 〈 0.05 ）. The fasting serum insulin level declined significantly （ P 〈0. 05 ） compared with model group, meanwhile, and the thyroid stimulating hormone level significantly increased （P 〈 0.05 ）, and SDF were more effective （ P 〈 0.05 ）. In addition, the hepatic Index and the hepatic lipid levels including TC and TG were decreased （P 〈 0.05 ）, while the activity of hepatic lipase （HL） and LPL were （P 〈0.05 ） increased. However, compared with normal control group, significant difference was indicated （P 〈 0.05 ）, and SDF was more effective. The present results showed that oat SDF and IDF were beneficial to anti -obesity with dose effects, but function channel was varied.