钩藤化学成分分析

Chemical Constituents of Uncariae Ramulus Cum Uncis

目的:研究茜草科植物钩藤的化学成分。方法:利用硅胶柱色谱,羟丙基葡聚糖凝胶,ODS,制备高效液相色谱法等方法对钩藤的乙醇提取物进行分离纯化,根据其理化性质与波谱数据对所分得的化合物进行结构鉴定。结果:从钩藤的乙醇提取物中分离得到14个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1),胡萝卜苷(daucosterol,2),5-O-咖啡酰基奎宁酸(chlorogenicacid,3),柯诺辛碱(corynoxine,4),去氢钩藤碱(corynoxeine,5),异去氢钩藤碱(isocorynoxeine,6),钩藤碱(rhynchophylline,7),异钩藤碱(isorhynchophylline,8),普鲁托品(protopine,9),卡丹宾碱(cadambine,10),二氢卡丹宾碱(3α-dihydrocadambine,11),喜果苷(vincoside lactam,12),异长春花苷内酰胺(strictosamide,13),金丝桃苷(hyperoside,14)。结论:化合物9为首次从该属植物中分离得到,化合物3,13,14为首次从该植物中分离得到。

Objective： To study the Chemical Constituents of Uncariae Ramulus Cum Uncis. Method： The compounds were obtained and purified by column chromatography on silica gel column, Sephadex LH-20, ODS and preparative HPLC, the structures of these compounds were identified according to their physicochemical properties and spectral data. Result： Fourteen compounds were obtained from the ethanol extract of Uncariae Ramulus Cum Uneis, their structures were elucidated as β-sitosterol （1） , daucosterol （2） , ehlorogenicacid （3） , corynoxine （4）, corynoxeine （5）, isocorynoxeine （6）, rhynchophylline （7）, isorhynchophylline （8） , protopine （9） , cadambine （10） , 3α-dihydrocadambine （ 11 ） , vincoside lactam （12） , strictosamide （13） , hyperoside （14）. Conclusion： Compound 9 was obtained from this genus for the first time. Compounds 3, 13 and 14 were obtained from the plant for the first time.