血府逐瘀汤中桔梗和牛膝对芍药苷组织分布的影响

Influence of Platycodonis Radix and Achyranthis Bidentatae Radix in Xuefu Zhuyu Tang on Tissue Distribution of Paeoniflorin

目的:比较桔梗和牛膝不同配伍对正常和血瘀小鼠体内芍药苷组织分布的影响,研究桔梗和牛膝的引经作用。方法:正常和血瘀小鼠分别灌胃血府逐瘀汤全方、单缺桔梗方、单缺牛膝方、缺桔梗和牛膝方,30 min后处死,收集全血,取出脑、心、肝、脾、肺、肾。采用HPLC测定芍药苷含量,流动相乙腈-水(16∶84),检测波长230 nm,栀子苷为内标物。结果:正常组小鼠分别灌胃血府逐瘀汤全方、单缺桔梗方、单缺牛膝方、缺桔梗和牛膝方后,脑、心、肝、脾中均未检测到芍药苷,肺中芍药苷质量分数分别为0.010 2,0.005 8,0.091 0,0.037 3μg·g-1,肾中芍药苷质量分数分别为0.122 2,0.052 0,0.144 0,0.065 0μg·g-1。血瘀小鼠灌胃血府逐瘀汤全方和单缺桔梗方后,脑中均未检测到芍药苷,心、肝、脾中均能检测到芍药苷,肺中芍药苷质量分数分别为0.076 4,0.042 0μg·g-1,肾中芍药苷质量分数分别为0.335,0.210μg·g-1;血瘀小鼠灌胃单缺牛膝方、缺桔梗和牛膝方后,脑、心、肝、脾中均几乎未检测到芍药苷,肺中芍药苷质量分数分别为0.274 7,0.019 7μg·g-1,肾中芍药苷质量分数分别为0.467 1,0.149 0μg·g-1。结论:基于缺桔梗和牛膝方比较,桔梗能增大肺、肾中芍药苷含量,对于血瘀小鼠,其增大肺中芍药苷含量的作用尤其显著;牛膝能减小正常小鼠肺、肾中芍药苷含量,能增大血瘀小鼠肺、肾中芍药苷含量,并使得芍药苷在心、肝、脾中分布。证实桔梗、牛膝载药至特定病所的的引经作用,且发现桔梗、牛膝的引经部位并不单一。

Objective： To compare effect of different compatibility of Platycodonis Radix and Achyranthis Bidentatae Radix on tissue distribution of paeoniflorin in normal and blood stasis mice, preliminary study leading action of Platycodonis Radix and Achyranthis Bidentatae Radix. Method： Normal and blood stasis mice were gavaged with Xuefu Zhuyu Tang, lacked Platycodonis Radix or Achyranthis Bidentatae Radix parties. Mice were killed after 30 minutes, whole blood collected, the brain, heart, liver, spleen, lung and kidney were removed. HPLC was employed to determine the content of paeoniflorin with mobile phase of acetonitrile-water （16： 84） ,detection wavelength at 230 nm and geniposide as internal standard. Result： After normal mice being gavaged with Xuefu Zhuyu Tang full party, single lacked Platycodonis Radix party, single lacked Achyranthis Bidentatae Radix party, lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, the content of paeoniflorin in lung were 0. 010 2, 0. 005 8, 0. 091 0, 0. 037 3 μg·g^-1, those in kidney were 0. 122 2, 0. 052 0, 0. 144 0, 0. 065 0 μg ·g^-1, but paeoniflorin were unchecked in the brain, heart, liver and spleen. After blood stasis mice being gavaged with Xuefu Zhuyu Tang full party, single lacked Platycodonis Radix party, the content of paeoniflorin in lung were 0. 076 4, 0. 042 0 μg ·g^-1, those in kidney were 0. 335 , 0. 210 μg ·g^-1 , but it was unchecked in the brain, heart, liver and spleen. After blood stasis mice being garaged with Xuefu Zhuyu Tangsingle lacked Achyranthis Bidentatae Radix party, lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, the content of paeoniflorin in lung were 0. 274 7, 0. 019 7 μg ·g^-1, those in kidney were 0. 467 1 , 0. 149 0 μg·g ^-1, it almost were unchecked in the brain, heart, liver and spleen. Conclusion： Making comparison based on Xuefu Zhuyu Tang lacked Platycodonis Radix and Achyranthis Bidentatae Radix party, Platycodonis Radix can increase the content of paeoniflorin in the lung and kidney in normal mice, Platycodonis Radix can more significantly increase the content of paeoniflorin in the lung than that in the kidney in blood stasis mice. Achyranthis Bidentatae Radix can decrease the content of paeoniflorin in the lung and kidney in normal mice, it can increase the content of paeoniflorin in the lung and kidney in blood stasis mice, it also can make distribution of paeoniflorin in the heart, liver, spleen in blood stasis mice. This study confirms Platycodonis Radix and Achyranthis Bidentatae Radix have effect of leading action. Leading sites of Platycodonis Radix and Achyranthis Bidentatae Radix are not single.