摘要
目的建立HPLC-MS/MS法测定川楝子中川楝素的方法。方法采用XTerra C18色谱柱（150mm×2.1mm,5μm）,以乙腈-0.2mL·L^-1甲酸溶液（31∶69）为流动相,采用多反应监测（MRM）模式进行质谱测定。结果川楝素检出限（LOD）为0.17ng,定量限（LOQ）为0.51ng;川楝素质量浓度在0.05-5.00μg·mL^-1范围内,与峰面积线性关系良好,r=0.999 9;精密度良好,平均回收率为98.0%,RSD为1.5%。结论该方法具有较好的专属性和灵敏度,能够简便、快速地测定川楝子药材中川楝素的含量。
Objective To establish a method of HPLC‐MS/MS for detecting toosendanin （TSN ） in Toosendan Fructus . Methods The analytes were isolated on a XTerra C18 column （150 mm × 2 .1 mm ,5 μm） by isocratic elution with acetonitrile‐mL · L^ -1formic acid（31∶ 69）as the mobile phase ,and detected by MS/MS in a multiple reaction monitoring （MRM） mode . Results LOD of TSN was 0 .17 ng ,and LOQ of TSN was 0 .51 ng ;The linear range of TSN concentration was 0 .05‐5 .00μg · mL^ -1 （r=0 .999 9）;The average recoveries were 98 .0% with RSD 1 .5% .Conclusion The method is simple and rapid with high sensitivity and good reproducibility ,and it could be used to determine the contents of TSN in Toosendan Fructus .
出处
《西北药学杂志》
CAS
2015年第5期572-575,共4页
Northwest Pharmaceutical Journal
