摘要
目的检测B7家族B7-H5以及CD28H在直肠癌组织中的表达情况。方法收集来我院治疗的78例结直肠癌患者的组织样本,通过免疫组化方法,以及实时定量PCR方法检测其B7-H5/CD28H表达情况。结果直肠癌组织部分B7-H5以及CD28H m RNA表达水平均高于癌旁组织(P<0.05)与正常组织(P<0.05),癌组织分组比较无显著差异(P>0.05),癌旁组织与正常组织无显著差异(P>0.05)。免疫组化方法检测B7-H5蛋白表达水平显示,癌旁组织,正常组织与癌组织阳性率分别为83.3%,74.3%和23.1%,与癌组织比较均具有差异(P<0.05),癌组织分组间无显著差异(P>0.05),癌旁组织与正常组织无显著差异(P>0.05)。结论不同于B7家族其他成员,结直肠癌组织中B7-H5/CD28H的表达量下降,可能与免疫逃逸有关,因此可以成为一个新的检测结直肠癌的标志物。
Objective To detect the B7 family B7-H5 and CD28H expression in colorectal cancer tissue.MethodsColected 78 cases of patients with colorectal cancer tissue samples from our hospital,used immunohistochemical method,and real-time quantitative polymerase chain reaction （PCR） to detect the B7-H5/CD28H expression.Results Rectal cancer tissue section B7-H5 and CD28H mRNA expression levels were higher than tissue adjacent to carcinoma（P〈 0.05）and normal group（P〈 0.05）,the cancer group was no significant difference（P〉 0.05）.Tissue adjacent to carcinoma and normal group there was no significant difference（P〉 0.05）. Immunohistochemical method detected B7-H5 protein expression levels,according to the tissue adjacent to carcinoma and normal tissues and the cancerous tissue positive rate were 83.3%,74.3% and 23.1%,compared with the cancer had significant difference（P〈 0.05）,carcinoma group（P〉 0.05）, no significant differences between tissue adjacent to carcinoma and normal group there was no significant difference（P〉 0.05）.Conclusion Different from other members of the family of B7,colorectal cancer tissues decreased the expression of B7-H5/CD28H,may be associated with immune escape,so it can become a new detect colorectal cancer markers.
出处
《中国卫生标准管理》
2015年第24期167-169,共3页
China Health Standard Management
基金
湖南省卫生厅科研基金课题(课题号:B2014-179)
课题名称:B7-H5/CD28H信号途径在结直肠癌中的表达及其对抗肿瘤免疫的影响
